Korean J Anesthesiol.  2012 May;62(5):461-467. 10.4097/kjae.2012.62.5.461.

The effects of hydrogen sulfide under sevoflurane administration against ischemia and reperfusion injury in isolated rat heart

• Lee DK
• Lim SH
• Kim NS

• KMID: 2243826
• DOI: http://doi.org/10.4097/kjae.2012.62.5.461

Abstract

BACKGROUND
Hydrogen sulfide (H2S) produces a protective effect against myocardial ischemia and reperfusion injury. Sevoflurane, which is used for anesthesia in cardiac problem patients, also has a protective effect. This study is designed to reveal the effects of H2S under sevoflurane using rat hearts.
METHODS
The hearts were Langendorff-perfused, subjected to 30 minutes ischemia and 60 minutes reperfusion. Group I was a control group. The other groups were pretreated for 15 minutes before ischemia as follows: 1.6% sevoflurane for group S; 18.5 microM H2S S for group H; and 1.6% sevoflurane and 18.5 microM H2S simultaneously for group HS. Hemodynamics and the infarct size were measured.
RESULTS
Group HS presented depressed hemodynamics during pretreatment. LV function in group HS achieved better recovery than group I after reperfusion. The infarct size of groups S, H and HS was smaller than group I, while there were no differences between groups S, H and HS.
CONCLUSIONS
Exogenous H2S did not enhance the preconditioning effects of sevoflurane. Rather, the results suggest that H2S under sevoflurane might depress hemodynamics.

Keyword

Heart; Hydrogen sulfide; In vitro; Ischemia; Reperfusion injury; Sevoflurane

MeSH Terms

Anesthesia
Animals
Heart
Hemodynamics
Humans
Hydrogen
Hydrogen Sulfide
Ischemia
Methyl Ethers
Myocardial Ischemia
Rats
Reperfusion
Reperfusion Injury
Hydrogen
Hydrogen Sulfide
Methyl Ethers