Korean J Aerosp Environ Med.  2004 Dec;14(4):135-141.

Effect of Antioxidant Vitamin Supplementation on Hyperbaric Oxygen Injury in Rats

• Jung CK
• Yoo BI
• Lee KY
• Kang CS

Abstract

BACKGROUND
Antioxidants vitamin C and vitamin E may protect against the toxic effect of oxygen free radicals that are preferentially produced after exposures to hyperbaric oxygen (HBO). This study investigated the effect of vitamin C and vitamin E on serum nitric oxide (NO) concentration and intercellular adhesion molecule-1 (ICAM-1) expression on lung after HBO exposure.
METHODS
Male Sprague-Dawley rats weighing 200 to 250 g were exposed to HBO at 3 ATA of 100% O2 for 3 hours. The experimental groups were given vitamin C (125 mg/day per rat) and/or vitamin E (50 mg/day per rat) orally, from 5 days prior to the HBO exposure to the day of sacrifice. Serum NO concentrations were determined by measuring NO end product nitrite by non-enzymatic Griess assay. Expression of ICAM-1 on lung was observed by immunohistochemical analysis.
RESULTS
The serum nitrite levels were significantly increased after HBO exposure and were higher at 24 hours after HBO exposure than at 0 h (P<0.05). The expression of ICAM-1 was weak immediately after HBO exposure and enhanced at 24 hours. There were no pronounced suppressive effects of vitamins on serum NO production and ICAM-1 expression induced by the 3 hours HBO exposure.
CONCLUSION
The 3 hours HBO exposure induces the serum NO production and ICAM-1 expression on lung. The short-term supplementation of vitamin C or/and E do not suppress the NO production and ICAM-1 expression on lung.

Keyword

Hyperbaric oxygenation; Nitric oxide; Intercelluar adhesion molecule-1; Antioxidants

MeSH Terms

Animals
Antioxidants
Ascorbic Acid
Free Radicals
Humans
Hyperbaric Oxygenation
Intercellular Adhesion Molecule-1
Lung
Male
Nitric Oxide
Oxygen*
Rats*
Rats, Sprague-Dawley
Vitamin E
Vitamins*
Antioxidants
Ascorbic Acid
Free Radicals
Intercellular Adhesion Molecule-1
Nitric Oxide
Oxygen
Vitamin E
Vitamins