J Korean Acad Conserv Dent.  2010 May;35(3):211-221. 10.5395/JKACD.2010.35.3.211.

The effect of the removal of chondroitin sulfate on bond strength of dentin adhesives and collagen architecture

• Kim JR
• Park SJ
• Choi GW
• Choi KK

• KMID: 2294347
• DOI: http://doi.org/10.5395/JKACD.2010.35.3.211

Abstract

Proteoglycan is highly hydrophilic and negatively charged which enable them attract the water. The objective of study was to investigate the effects of Proteoglycan on microtensile bond strength of dentin adhesives and on architecture of dentin collagen matrix of acid etched dentin by removing the chondroitin sulphate attached on Proteoglycan. A flat dentin surface in mid-coronal portion of tooth was prepared. After acid etching, half of the specimens were immersed in 0.1 U/mL chondroitinase ABC (C-ABC) for 48 h at 37degrees C, while the other half were stored in distilled water. Specimens were bonded with the dentin adhesive using three different bonding techniques (wet, dry and re-wet) followed by microtensile bond strength test. SEM examination was done with debonded specimen, resin-dentin interface and acid-etched dentin surface with/without C-ABC treatment. For the subgroups using wet-bonding or dry-bonding technique, microtensile bond strength showed no significant difference after C-ABC treatment (p > 0.05). Nevertheless, the subgroup using rewetting technique after air dry in the Single Bond 2 group demonstrated a significant decrease of microtensile bond strength after C-ABC treatment. Collagen architecture is loosely packed and some fibrils are aggregated together and relatively collapsed compared with normal acid-etched wet dentin after C-ABC treatment. Further studies are necessary for the contribution to the collagen architecture of noncollagenous protein under the various clinical situations and several dentin conditioners and are also needed about long-term effect on bond strength of dentin adhesive.

Keyword

Chondroitin Sulphate; Collagen Architecture; Dentin Adhesive; Noncollagenous Protein; Microtensile Bond Strength

MeSH Terms

Adhesives
Bisphenol A-Glycidyl Methacrylate
Chondroitin
Chondroitin ABC Lyase
Chondroitin Sulfates
Collagen
Dentin
Proteoglycans
Tooth
Water
Adhesives
Bisphenol A-Glycidyl Methacrylate
Chondroitin
Chondroitin ABC Lyase
Chondroitin Sulfates
Collagen
Proteoglycans
Water

Figure

• 

  Figure 1 Results of microtensile bond strength value (MPa) of each dentin adhesives. W: wet dentin not treated with enzyme (C-ABC) was blot dried. D: dentin not treated with enzyme (C-ABC) was air dried for 15 s. RW: dentin not treated with enzyme (C-ABC) was air dried for 15 s and then re-wetted for 30 s with wet gauze and blot dried. E-W: wet dentin treated with enzyme (C-ABC) was blot dried. E-D: dentin treated with enzyme (C-ABC) was air dried for 15 s. E-RW, dentin treated with enzyme (C-ABC) was air dried for 15 s and then re-wetted for 30 s with wet gauze and blot dried.

• 

  Figure 2 Field Emission-Scanning Electron Microscope (FE-SEM) observations of the fractured surfaces in dentin side after microtensile bond strength testing (Mag: ×10,000). a. Dentin side produced by Single bond 2 without C-ABC treatment. Failure was occurred within the adhesive resin to the bottom of the hybrid layer. Dentinal tubules were occluded with the resin tag. b. Dentin side produced by Single bond 2 with C-ABC treatment. Failure was occurred at bottom of hybrid layer with resin tag exclusion. c. Dentin side produced by One Step Plus without C-ABC treatment. Failure was occurred from the top of the hybrid layer to the bottom. The hybrid layer is densely formed. d. Dentin side produced by One Step Plus with C-ABC treatment. Failure was occurred at bottom of hybrid layer with resin tag exclusion.

• 

  Figure 3 Field Emission-Scanning Electron Microscope (FE-SEM) observations of the resin-dentin interfaces (Mag: ×2,000). a. Photomicrograph of resin-dentin interface of Single Bond 2 without C-ABC treatment. Hybrid layer of about 6µm thickness is observed. b. Photomicrograph of resin-dentin interface of Single Bond 2 with CABC treatment. A thicker hybrid layer about 10µm thickness can be seen. c. Photomicrograph of resin-dentin interface of One Step Plus without C-ABC treatment. Hybrid layer of about 4~6µm thickness is observed. d. Photomicrograph of resin-dentin interface of One Step Plus with CABC treatment. A thicker hybrid layer about 10µm thickness can be seen like Single bond 2.

• 

  Figure 4 Field Emission-Scanning Electron Microscope (FE-SEM) observations of acid-etched dentin surfaces. a. Collagen fibril architecture of acid-etched wet dentin without C-ABC pre-treatment. b. A high-magnification view. Well-defined collagen fibrils are arranged with compact interfibrillar space. c. Collagen fibril architecture of acid-etched wet dentin with C-ABC pre-treatment. d. A high-magnification view. Collagen architecture is loosely packed with irregular diameter and some fibrils are fused together.

• 

  Figure 5 Field Emission-Scanning Electron Microscope (FE-SEM) observations of the vertically fractured dentin surface. a. Collagen fibrils vertical architecture of acid-etched wet dentin without C-ABC treatment. Collagen network is fully expanded state. b. Collagen fibrils vertical architecture of acid-etched wet dentin with C-ABC treatment. The collagen network is slightly collapsed compared with wet dentin without C-ABC treatment. c. Collagen fibrils vertical architecture of acid-etched re-wetted dentin with C-ABC pre-treatment. Collagen network is relatively collapsed compared with normal acid-etched wet dentin.

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