Korean J Urol.  2003 May;44(5):490-494.

Suppressive Effect on Renal Inflammation by Vitamin A Combined with Antibiotics in a Rat Model of Pyelonephritis

• Yun TK
• Lee SJ
• Cho YH

Abstract

PURPOSE: We evaluated the suppressive effects of vitamin A, in combination with antibiotics, on the development of inflammation in an animal model of ascending pyelonephritis.
MATERIALS AND METHODS
An inoculum, of 1x10(9) Escherichia coli (O2:K1:H(-) ) P69 organisms per ml, was instilled into rat bladders, and the urethras of each rat occluded for 4 hours. The individual treatments with ciprofloxacin (15mg/kg, twice a day for 5 days) or vitamin A (500 microgram/kg, once a day for 5 days), or a combination of the was initiated 72 hours after the bacterial inoculation. The rats' renal weights were measured and examinations of the bacterial culture performed. The kidneys were harvested 4 weeks post-infection, and assessed for histopathologic inflammation.
RESULTS
The renal weights in all groups were decreased, with the exception of the combined treatment group (p<0.05). In the urine and kidney microbiological cultures there were no E.coli growths in either the antibiotic or combined treatment groups. From microscopic examinations, the antibiotic treatment of pyelonephritic rats, in combination with vitamin A, was shown to result in significantly less renal inflammation compared to the other experimental groups.
CONCLUSIONS
Vitamin A therapy in combination with antiobiotics can significantly reduce the inflammation associated with ascending pyelonephritis.

Keyword

Vitamin A; Pyelonephritis; Inflammation

MeSH Terms

Animals
Anti-Bacterial Agents*
Ciprofloxacin
Escherichia coli
Inflammation*
Kidney
Models, Animal*
Pyelonephritis*
Rats*
Urethra
Urinary Bladder
Vitamin A*
Vitamins*
Weights and Measures
Anti-Bacterial Agents
Ciprofloxacin
Vitamin A
Vitamins