J Clin Pathol Qual Control.  1999 Dec;21(2):321-327.

Basic Evaluation of ADVIA 120 Hematology System

• Park H
• Moon KH
• Kim G

Abstract

BACKGROUND: ADVIA 120 Hematology System (Bayer Corporation, Tarrytown, NY, USA) is an autoanalyzer for hematology using mainly light scattering as a means of measuring. This study was carried out to evaluate proficiency for routine complete blood count (CBC) with white blood cell (WBC) differentials and reticulocyte counting of the instrument.
METHODS
The evaluation protocol was based on the recommendation of the ICSH (International Committee for Standardization in Haematology). Precision, linearity, carryover, sample preservation effect, comparability for CBC with WBC differentials and reticulocyte counting.
RESULTS
For precision, all parameters investigated showed the coefficient of variation (CV) values similar to or lower than those of manufacturer's. The storage effect was not significant till 6 hours after sampling but the significant change was observed after that at room temperature. To store samples before analysis for more than 6 hours, it should be stored at 4degrees C. The Linearity was excellent for WBC, red blood cell (RBC), hemoglobin and platelet with R2 over 0.985. Carryover was lower than that of manufacturer's with less than 0.28% for WBC, RBC, hemoglobin, platelet and reticulocyte. ADVIA 120 and Cell-dyn 4000 (Abbott Laboratories, IL., USA) showed good correlation especially in parameters such as WBC, RBC, hemoglobin, platelet, and red cell distribution width (RDW). Reasonably good precision, carryover and linearity was found in reticulocyte counting.
CONCLUSIONS
ADVIA 120 Hematology System showed excellent precision, linearity, and comparability and low carryover in the parameters evaluated to be a good instrument for routine hematology laboratory work including CBC with WBC differentials and reticulocyte counting.

MeSH Terms

Blood Cell Count
Blood Platelets
Erythrocyte Indices
Erythrocytes
Hematology*
Leukocytes
Reticulocyte Count
Reticulocytes