J Breast Cancer.  2013 Sep;16(3):285-290. 10.4048/jbc.2013.16.3.285.

Cytogenetic Finding of Breast Cancer Cases and in Their First-Degree Relatives

• Celik DA
• Kosar PA
• Ozcelik N
• Eroglu E

• KMID: 2286379
• DOI: http://doi.org/10.4048/jbc.2013.16.3.285

Abstract

PURPOSE
The aim of this study was to evaluate and compare the rate of sister chromatid exchange (SCE), the occurrence of micronuclei, and the lymphocyte proliferation rate index (PRI) in patients with breast cancer, their first-degree relatives, and healthy volunteers.
METHODS
We analyzed the frequency of SCE and micronuclei, and the PRI in the peripheral blood lymphocytes of 30 women with breast cancer, 22 of their female family members, and 20 age-matched healthy female volunteers.
RESULTS
SCE occurred significantly more often in the lymphocytes of breast cancer patients (10.84+/-0.4 per metaphase), compared with their first-degree relatives (7.45+/-0.54) and controls (5.94+/-0.2) (p<0.001 for both). The mean SCE frequency was not statistically different between first-degree relatives and controls (p=0.071). Similarly, micronuclei occurred at a significantly higher rate in breast cancer patients (9.6+/-0.72), and in their first-degree relatives (7+/-0.64), compared to controls (3.85+/-0.4) (p<0.001 and p=0.001, respectively). There was also a significant difference between the occurrence of micronuclei in patients compared to their family members (p=0.021). The PRI was significantly lower in patients (1.61+/-0.1), compared with both their first-degree relatives (1.75+/-0.1), and controls (1.74+/-0.1) (p=0.001 and p=0.002, respectively).
CONCLUSION
Increased SCE and the occurrence of micronuclei, as well as a reduced PRI are associated with breast cancer. Furthermore, increased SCE and the frequency of micronuclei in a first-degree relative suggest that they exhibit greater genetic instability than women of the same age.

Keyword

Breast neoplasms; DNA damage; Germline micronucleus; Sister chromatid exchange

MeSH Terms

Breast
Breast Neoplasms
Cytogenetics
DNA Damage
Female
Humans
Lymphocytes
Micronucleus, Germline
Sister Chromatid Exchange

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