J Asthma Allergy Clin Immunol.
1999 Dec;19(6):912-919.
Spontaneous expression of mRNA for IL - 10, GM - CSF and TNF - alpha in peripheral blood mononuclear cells from patients with atopic dermatitis
Abstract
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BACKGROUND: Monocytes and T helper cells play major roles in the immunologic dysfunction
of atopic dermatitis (AD). Many studies have been done on the cytokine pattern to evaluate
abnormalities or differences of immune cells in AD, but the results were conflicting
among studies and most of these previous reports were performed with various kinds of
mitogen-stimulation.
OBJECTIVE
The purpose of this study was to investigate spontaneous cytokine pattern in peripheral blood mononuclear cells (PBMC) from patients with AD. We focused on the expression of monokines that had effects on monocytes and T cells.
METHODS
We measured mRNA expression of IL-10, GM-CSF and TNF-alpha in freshly isolated PBMC with semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The intensity of cytokine cDNA was normalized to that of beta-actin product as a standard marker.
RESULTS
IL-10 mRNA expression was significantly enhanced in AD compared:with control subjects (p<0.05). Spontaneous mRNA expression of TNF-alpha was significantly lower in AD patients (p <0.01). The level of GM-CSF mRNA expression was heterogeneous and spontaneous mRNA expression was slightly increased in AD although the difference did not reach the level of statistical significance.
CONCLUSION
Our data was able to represent in vivo cytokine expression state of PBMC in atopic dermatitis. Increased expression of IL-10 and GM-CSF may have been associated with monocyte dysfunction in AD although increase in the expression of GM-CSF mRNA was not statistically significant. TNF-alpha mRNA expression was decreased in AD and increased IL-10 was suggested to exert an inhibitory effect on the expression of TNF-alpha mRNA.
MeSH Terms
-
Actins
Dermatitis, Atopic*
DNA, Complementary
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Interleukin-10
Monocytes
Monokines
RNA, Messenger*
T-Lymphocytes
T-Lymphocytes, Helper-Inducer
Tumor Necrosis Factor-alpha
Actins
DNA, Complementary
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-10
Monokines
RNA, Messenger
Tumor Necrosis Factor-alpha
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