Korean J Physiol Pharmacol.  2014 Apr;18(2):183-190. 10.4196/kjpp.2014.18.2.183.

Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice

• Li GJ
• Sun P
• Wang R
• Zhou YL
• Qian Y
• Zhao X

• KMID: 2285509
• DOI: http://doi.org/10.4196/kjpp.2014.18.2.183

Abstract

This project's aim was to determine the reserpine-induced gastric ulcer preventive effect of polysaccharide of Larimichthys crocea swim bladder (PLCSB) in ICR mice. The anti-gastric ulcer effects of polysaccharide of Larimichthys crocea swim bladder was evaluated in mice model using morphological test, serum levels assay, cytokine levels assay, tissue contents analysis, reverse transcription-polymerase chain reaction (RT-PCR) analysis and western bolt assay. High concentration (50 mg/kg dose) of PLCSB reduced IFN-gamma as compared to low concentration (25 mg/kg dose) and control mice. SS and VIP serum levels of PLCSB treated mice were higher than those of control mice, and MOT and SP serum levels were lower than control mice. Gastric ulcer inhibitory index of PLCSB treatment groups mice were much lower than control mice, and the high concentration treated mice were similar to the ranitidine treated mice. The SOD and GSH-Px activities of PLCSB treated mice were higher than control mice, close to normal mice and ranitidine treated mice. PLCSB treated mice also showed the similar contents of NO and MDA to normal group. By RT-PCR and western blot assay, PLCSB significantly induced inflammation in tissues of mice by downregulating NF-kappaB, iNOS, and COX-2, and upregulating IkappaB-alpha . These results suggest that PLCSB showed a good gastric ulcer preventive effect as the gastric ulcer drug of ranitidine. Polysaccharide of Larimichthys crocea swim bladder may be used as a drug material from marine products.

Keyword

Cytokine; Gastric ulcer; ICR mice; Larimichthys crocea swim bladder; Polysaccharide

MeSH Terms

Animals
Blotting, Western
Inflammation
Mice
Mice, Inbred ICR*
NF-kappa B
Ranitidine
Reserpine*
Stomach Ulcer*
Ulcer
Urinary Bladder*
NF-kappa B
Ranitidine
Reserpine

Figure

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  Fig. 1 Stomachs of the mice treated with polysaccharide of Larimichthys crocea swim bladder (PLCSB) after the induction of gastric ulcer with reserpine.

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  Fig. 2 Gastric secretion volume of the mice treated with polysaccharide of Larimichthys crocea swim bladder (PLCSB) after the induction of gastric ulcer with reserpine. a~dMean values with different letters over the bars are significantly different (p<0.05) according to Duncan's multiple range test.

• 

  Fig. 3 Serum IL-6 (A), IL-12 (B), TNF-α (C) and IFN-γ (D) cytokine levels of reserpine-induced gastric ulcer mice treated with polysaccharide of Larimichthys crocea swim bladder (PLCSB). a~dMean values with different letters over the bars are significantly different (p<0.05) according to Duncan's multiple range test.

• 

  Fig. 4 Serum MOL (motillin) (A), SS (somatostatin) (B), SP (substance P) (C) and VIP (vasoactive intestinal peptide) (D) levels of reserpine- induced gastric ulcer mice treated with polysaccharide of Larimichthys crocea swim bladder (PLCSB). a~eMean values with different letters over the bars are significantly different (p<0.05) according to Duncan's multiple range test.

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  Fig. 5 Tissue SOD (superoxide dismutase), GSH-Px (glutathione peroxidase), NO (nitrogen oxide) and MDA (maleic dialdehyde) contents of reserpine-induced gastric ulcer mice treated with polysaccharide of Larimichthys crocea swim bladder (PLCSB). a~eMean values with different letters over the bars are significantly different (p<0.05) according to Duncan's multiple range test.

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  Fig. 6 Effects of polysaccharide of Larimichthys crocea swim bladder (PLCSB) on the mRNA and protein expression of NF-κB, IκB-α, iNOS and COX-2 in reserpine-induced gastric ulcer mice.

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