Korean J Physiol Pharmacol.  2004 Jun;8(3):141-146.

Alteration of Immunoreactivity for SNARE Proteins in the Rat Hippocampus after Middle Cerebral Artery Occlusion

• Park JS
• Huh PW
• Jung YJ
• Park SJ
• Lee KE

Abstract

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins, composed of two presynaptic membrane proteins [synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin] and a presynaptic vesicular protein [vesicle-associated membrane protein (VAMP) ], serve as a core of exocytotic fusion machinery, which can be affected by ischemia. Synaptic protein in core region, striatum and cortex has been shown to alter after focal ischemia, however, little is known in hippocampus. Hippocampus is remote from ischemic core, but it is one of the most vulnerable regions. Using immunohistochemistry, the present study was undertaken to investigate the alteration of expression of SNAP-25, syntaxin, and VAMP in the hippocampus of rats which were subjected to middle cerebral artery occlusion (MCAO) for 2h and allowed to reperfuse. At 2 weeks of reperfusion, the SNAP-25 and syntaxin immunoreactivity was increased in the stratum oriens of the CA1 and the stratum lucidum of the CA3 in the ipsilateral hippocampus. However, VAMP immunoreactivity didn't show significant change. These results demonstrate that the level of the presynatpic plasma membrane proteins (SNAP-25 and syntaxin) in the rat hippocampus is more sensitively affected by focal ischemia than that of the synaptic vesicle protein (VAMP).

Keyword

Middle cerebral artery occlusion; Hippocampus; SNAP25; Syntaxin; VAMP

MeSH Terms

Animals
Cell Membrane
Hippocampus*
Immunohistochemistry
Infarction, Middle Cerebral Artery*
Ischemia
Membrane Proteins
Middle Cerebral Artery*
Qa-SNARE Proteins
Rats*
Reperfusion
SNARE Proteins*
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Synaptic Vesicles
Membrane Proteins
Qa-SNARE Proteins
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins