Infect Chemother.  2005 Feb;37(1):22-28.

VIM-2 Type Metallo-beta-lactamase Producing Achromobacter xylosoxidans subsp. xylosoxidans Isolated from Urine Specimens

• Shin KS
• Lee J
• Kim BC

Abstract

BACKGROUND: The dissemination of metallo-beta-lactamase (MBL) producing gram-negative bacilli is of great concern because MBL can hydrolyze carbapenem. We report herein the infection by VIM-2 type MBL producing Achromobacter xylosoxidans subsp. xylosoxidans.
MATERIALS AND METHODS
For seven A. xylosoxidans subsp. xylosoxidans with reduced imipenem susceptibility, the detection for MBL was performed using EDTA double disk synergy test (EDTA- DDS) and the PCR for IMP-1, VIM-1 and VIM-2 genes. The minimal inhibitory concentration (MIC) of MBL producers were determined by microbroth dilution methods. The DNA fingerprinting analysis was performed by random amplified polymorphic DNA.
RESULTS
All seven isolates were MBL producers when tested with EDTA-DDS test and these isolates were VIM-2 type confirmed by the PCR and DNA sequencing analysis. The MIC against imipenem ranged from 16 to 32 microgram/mL in these isolates. The DNA fingerprints of these isolates were identical.
CONCLUSION
A. xylosoxidans subsp. xylosoxidans manifest resistance against imipenem by acquisition of VIM-2 type MBL. To our knowledge, this is the first report on the VIM-2 type MBL producing A. xylosoxidans subsp. xylosoxidans.

Keyword

Achromobacter xylosoxidans subsp. xylosoxidans; VIM-2; Metallo-beta-lactamase; Carbapenem; RAPD

MeSH Terms

Achromobacter denitrificans*
Achromobacter*
DNA
DNA Fingerprinting
Edetic Acid
Imipenem
Polymerase Chain Reaction
Sequence Analysis, DNA
DNA
Edetic Acid
Imipenem