Korean J Prev Med.  1984 Sep;17(1):245-250.

Sister Chromatid Exchanges in Lymphocytes on Normal Human Blood culture with Mercury chloride or Methylmercury Chloride

Affiliations
  • 1Department of Preventive Medicine and Public Health, Chonbug National University Medical School, Korea.

Abstract

Reciprocal exchanges of DNA in sister chromatids (SCEs) are induced by various carcinogens and mutagens, although the quantitative relationship between the number of mutations and SCEs induced varies among chemicals. Nevertheless, the analysis of SCEs production by various agents often proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity. Mercury, even if which has no evidences for mutagenicity and carcinogenicity, is reported to exert women cytotoxic effects, such as chromosomal aberrations or bad influences to ovulation and reproduction in experimental animals, etc. In this study, tests for sister chromatid exchanges have been carried out on normal human lymphocytes in whole blood culture to add mercury chloride (HgCl2) or methylmercury chloride(CH3 HgCl) for 72 hr. The results indicate the dose-dependent relationship between the frequencies of SCEs and the concentrations of HgCl2, CH3HgCl and 5-bromo-2-deoxyuridine (BrdU). Lymphocyte proliferation has depressed in the higher concentration of mercury.


MeSH Terms

Animals
Carcinogens
Chromatids
Chromosome Aberrations
DNA
Female
Humans
Humans*
Lymphocytes*
Mercuric Chloride
Mutagens
Ovulation
Reproduction
Siblings*
Sister Chromatid Exchange*
Carcinogens
DNA
Mercuric Chloride
Mutagens
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