Diabetes Metab J.  2011 Apr;35(2):149-158. 10.4093/dmj.2011.35.2.149.

Changes in Adenosine Deaminase Activity in Patients with Type 2 Diabetes Mellitus and Effect of DPP-4 Inhibitor Treatment on ADA Activity

• Lee JG
• Kang DG
• Yu JR
• Kim YR
• Kim JS
• Koh GP
• Lee DH

• KMID: 2281713
• DOI: http://doi.org/10.4093/dmj.2011.35.2.149

Abstract

BACKGROUND
Dipeptidyl peptidase 4 (DPP-4, also known as CD26) binds with adenosine deaminase (ADA) to activate T lymphocytes. Here, we investigated whether ADA activity is specifically affected by treatment with DPP-4 inhibitor (DPP4I) compared with other anti-diabetic agents.
METHODS
Fasting ADA activity, in addition to various metabolic and biochemical parameters, were measured in 262 type 2 diabetes mellitus (T2DM) patients taking various anti-diabetic agents and in 46 non-diabetic control subjects.
RESULTS
ADA activity was increased in T2DM patients compared with that in non-diabetic control subjects (mean+/-standard error, 23.1+/-0.6 U/L vs. 18.6+/-0.8 U/L; P<0.05). ADA activity was correlated with fasting plasma glucose (r=0.258, P<0.05), HbA1c (r=0.208, P<0.05), aspartate aminotransferase (r=0.325, P<0.05), and alanine aminotransferase (r=0.248, P<0.05). Compared with the well-controlled T2DM patients (HbA1c<7%), the poorly controlled group (HbA1c>9%) showed significantly increased ADA activity (21.1+/-0.8 U/L vs. 25.4+/-1.6 U/L; P<0.05). The effect of DPP4I on ADA activity in T2DM patients did not differ from those of other oral anti-diabetic agents or insulin. T2DM patients on metformin monotherapy showed a lower ADA activity (20.9+/-1.0 U/L vs. 28.1+/-2.8 U/L; P<0.05) compared with that of those on sulfonylurea monotherapy.
CONCLUSION
Our results show that ADA activity is increased in T2DM patients compared to that in non-diabetic patients, is positively correlated with blood glucose level, and that DPP4I has no additional specific effect on ADA activity, except for a glycemic control- or HbA1c-dependent effect.

Keyword

Adenosine deaminase; Diabetes mellitus, type 2; Dipeptidyl peptidase

MeSH Terms

Adenosine
Adenosine Deaminase
Alanine Transaminase
Aspartate Aminotransferases
Blood Glucose
Diabetes Mellitus, Type 2
Dipeptidyl Peptidase 4
Fasting
Glucose
Humans
Insulin
Metformin
Plasma
T-Lymphocytes
Adenosine
Adenosine Deaminase
Alanine Transaminase
Aspartate Aminotransferases
Blood Glucose
Dipeptidyl Peptidase 4
Glucose
Insulin
Metformin