Korean J Pain.  2012 Oct;25(4):238-244. 10.3344/kjp.2012.25.4.238.

Antinociceptive Effects of Intraperitoneal and Intrathecal Vitamin E in the Rat Formalin Test

Affiliations
  • 1Department of Anesthesiology and Pain Medicine, Chungnam National University Hospital, Daejeon, Korea. ane84@cnu.ac.kr

Abstract

BACKGROUND
Vitamin E is widely known to be one of the reactive oxygen species (ROS) scavengers and a drug that can easily be obtained, and it has been shown to attenuate the pain responses induced by various causes in animal pain models. Thus, this experiment was conducted to assess the antinociceptive effects of vitamin E by comparing intraperitoneal and intrathecal injections in rats subjected to the formalin test.
METHODS
After the intraperitoneal and intrathecal injections of vitamin E were carried out, respectively (IP: 500 mg/kg, 1 g/kg, and 2 g/kg, IT: 3 mg/kg, 10 mg/kg, and 30 mg/kg), the formalin test was perfumed. As soon as 5% formalin was injected into left hind paw, the number of flinches induced by pain was measured at 5-minute intervals for 1 hour.
RESULTS
Formalin injected into the left hind paw induced biphasic nociceptive behavior in all animals. Intraperitoneal injection of vitamin E diminished the nociceptive behavior in a dose-dependent manner during the early and late phase. Intrathecal vitamin E diminished nociceptive behavior dose dependently during the late phase but showed no significant difference in the early phase.
CONCLUSIONS
Vitamin E attenuated acute nociception when it was injected systemically, while both systemic and intrathecal injection produced analgesia in a rat model of formalin-induced hyperalgesia.

Keyword

formalin test; intraperitoneal; intrathecal; vitamin E

MeSH Terms

Analgesia
Animals
Formaldehyde
Hyperalgesia
Injections, Intraperitoneal
Injections, Spinal
Nociception
Pain Measurement
Rats
Reactive Oxygen Species
Vitamin E
Vitamins
Formaldehyde
Reactive Oxygen Species
Vitamin E
Vitamins
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