Korean J Pediatr Gastroenterol Nutr.  2011 Sep;14(3):269-278. 10.5223/kjpgn.2011.14.3.269.

Tumor Necrosis Factor-alpha Gene Polymorphisms in Korean Children with Inflammatory Bowel Disease

• Cho MS
• Song SM
• Oh SH
• Lee YJ
• Jang JY
• Kim KM

• KMID: 2276886
• DOI: http://doi.org/10.5223/kjpgn.2011.14.3.269

Abstract

PURPOSE
The aim of this study was to investigate the existence of TNF-alpha polymorphisms in Korean children with Crohn's disease (CD), ulcerative colitis (UC), as compared to healthy controls.
METHODS
Blood samples were obtained from 40 patients with CD, 14 patients with UC, and 30 healthy controls. Genotyping for 5 TNF-alpha polymorphisms (G238A, G308A, C857T, C863A, and T1031C) was performed. The allele frequencies for the inflammatory bowel diseases, CD and UC, were measured in patients with these disease and in healthy controls, and these allele frequencies were compared between the 3 groups. We examined the significant association between polymorphism and disease phenotype, such as location, behavior, perianal disease, and pediatric Crohn's activity index (PCDAI) in CD.
RESULTS
Based on our findings, the TNF-alpha allele frequencies of G238A, G308A, C857T, C863A, and T1031C were 3.3, 8.3, 16.7, 16.7, 21.7% in healthy control, 2.5%, 7.5%, 18.8%, 20.0%, 22.5% in CD, 7.1%, 7.1%, 7.1%, 21.4%, 28.6% in UC. They were no statistically significant differences between the 3 groups. There were no associations between genotypes and phenotypes in CD, except a statistically significant higher allele frequency of G238A in ileal type (L1) disease (p=0.010).
CONCLUSION
Our results indicate that 5 TNF-alpha polymorphisms do not seem to be associated with susceptibility to inflammatory bowel disease in Korean pediatric patients even though young patients were anticipated to have a stronger genetic affiliation for these diseases than adult patients. We think that further studies are needed to find those genes associated with susceptibility to CD and UC in Korean pediatric patients with inflammatory bowel disease.

Keyword

Crohn's disease; TNF-alpha; Gene polymorphism; Ulcerative colitis

MeSH Terms

Adult
Child
Colitis, Ulcerative
Crohn Disease
Gene Frequency
Genotype
Humans
Inflammatory Bowel Diseases
Phenotype
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor-alpha

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