Chonnam Med J.  2002 Dec;38(4):307-314.

Modulatory Effects of Ginsenoside on the Regulation of KATP Channel Activity in Rat Myocardium

• Ju JM
• Lee YJ
• Park JS
• Kim JH

Abstract

Modulatory effects of protopanaxadiol (PPD), a group of the extracted ginsenosides, on the regulation of ATP-sensitive potassium channel (KATP channel) activity were examined, using patch clamp technique, in isolated rat ventricular myocytes. In excised inside-out patch, PPD reduced KATP channel activities in a dose-dependent manner, and both the frequency of channel opening and the mean open-burst duration were markedly decreased, but the single channel current amplitude was not affected. The conductance slopes obtained from the current-voltage (I-V) relationship curves of the PPD-treated (100 and 300 micro gram/ml) groups were not significantly different from that of the non-treated control group. PPD did not affect the channel activities when the channel activities were reduced by the application of 100 M ATP to the bath. In cell-attached patch, PPD (100 micro gram/ml) attenuated the dinitrophenol (50micro M)-induced KATP channel activities. These results suggest that the ginsenoside is involved in the regulation of KATP channel activities in the rat myocardium, but those inhibitory effects on the KATP channel activities could not be benificial for the ischemic myocardium.

Keyword

Ginsenoside; KATP channel; Rat; Myocardium; Patch clamp

MeSH Terms

Adenosine Triphosphate
Animals
Baths
Ginsenosides
Muscle Cells
Myocardium*
Potassium Channels
Rats*
Adenosine Triphosphate
Ginsenosides
Potassium Channels