Korean J Otolaryngol-Head Neck Surg.  1997 Jun;40(6):835-841.

Role of p53, Ras p21 and HPV Type 16, 18 on Malignant Transformaton in Inverted Papilloma of Nose and Paranasal Sinus

• Byun JY
• Cho JS
• Lee BJ
• Lee JG
• shin SS
• Ahn HY

Abstract

Mutation in p53 and p21 genes is the most common specific genetic change found in human cancers and has been investigated in a great number of neoplastic lesion. There have been some reports dealing with relationship between human papillomavirus(HPV) and papilloma associated carcinoma. But the roles of p53, ras oncoprotein and HPV have not been fully described in malignant transformation of inverted papilloma. The aim of this study was to demonstrate the mutated oncogenes immunohistochemically and evaluate relationship between expression of the oncogenes and HPV. The materials compromised 13 cases of the inverted papilloma and 6 cases associated with carcinoma. Immunohistochemical staining for p53 and ras oncogene was performed and molecular study of HPV DNA type16 and 18 was also performed with PCR on the paraffin embedded material. The carcinomatous and dysplastic areas were diffusely positive for p53 in 5 of 6 cases associated with carcinoma, whereas control group was negative for p53. And p21 immunohistochemical staining was diffusly positive in both group. A HPV DNA type 16 was negative, but type 18 was positive in 2 cases associated with carcinoma. The results indicated that p53 and HPV type 18 may be involved in pathogenesis of malignant transformation in inverted papilloma.

Keyword

Malignant changed sinonasal inverted papilloma; p53; Ras p21; Human papilloma virus

MeSH Terms

DNA
Genes, ras
Humans
Nose*
Oncogenes
Papilloma
Papilloma, Inverted*
Paraffin
Polymerase Chain Reaction
DNA
Paraffin