Korean J Obstet Gynecol.  2008 Feb;51(2):199-211.

The effect of GM-CSF on the expression of implantation-related genes in mouse embryo

• Kim HJ
• Kim HM
• Kim JL
• Shin JH
• Hong SY
• Park EJ
• Park WI
• Lee JY
• Cha BH
• Moon HS

Abstract

OBJECTIVE
GM-CSF is produced in female reproductive tract and may play an important role in the process of implantation. Body of evidence suggests that GM-CSF could improve pregnancy rate in many species of mammals when it was added in culture media. The aim of this study is to assess how GM-CSF affects the expression of implantation-related genes in mouse embryo.
METHODS
Two hundred mouse embryos were divided into control and GM-CSF treated groups. The embryos were treated with or without 10 ng/ml of GM-CSF for 72 hours. Total RNA was isolated and compared with oligo microarray. The implantation-related genes influenced by GM-CSF were repeatedly analyzed by real-time PCR.
RESULTS
After oligo microarray, 64 genes were increased and 35 genes were decreased by GM-CSF. Among those genes, MMP2, FABP3, Dppa5 and TAS1 were selected for real-time PCR analysis. Four integrins and FAK were also selected. We confirmed the increase of MMP2 and FABP3 by GM-CSF with real-time RT-PCR (1.687 and 1.580 fold, respectively). Other genes were found to be minimally increased.
CONCLUSION
GM-CSF induces the increased expression of MMP2 mRNA in mouse embryo, and then increases the invasiveness of the trophoblast. The role of FABP3 in the process of implantation remained to be elucidated.

Keyword

Embryo; Implantation; GM-CSF; MMP2; FABP3

MeSH Terms

Animals
Culture Media
Embryonic Structures
Female
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Integrins
Mammals
Mice
Pregnancy Rate
Real-Time Polymerase Chain Reaction
RNA
RNA, Messenger
Trophoblasts
Culture Media
Granulocyte-Macrophage Colony-Stimulating Factor
Integrins
RNA
RNA, Messenger