Korean J Fertil Steril.  2001 Jun;28(2):161-168.

The Effect of GM-CSF Supplementation in Culture Medium in the Human IVF Programs

• Park WI
• Kwon HC
• Kim DH
• Kang HK
• Kim MK
• Lee HC
• Jung JH
• Lee MS
• Lee HJ

Abstract

OBJECTIVE
Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate.
METHODS
A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control =96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared.
RESULTS
There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively).
CONCLUSION
By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved.

Keyword

GM-CSF; Embryo; IVF

MeSH Terms

Colony-Stimulating Factors
Embryonic Development
Embryonic Structures
Female
Fertilization
Gonadotropins
Granulocyte-Macrophage Colony-Stimulating Factor*
Humans*
Oocytes
Pregnancy
Sperm Injections, Intracytoplasmic
Colony-Stimulating Factors
Gonadotropins
Granulocyte-Macrophage Colony-Stimulating Factor