Interaction between Causative Factors of Cervical Cancer and BARD-1 (BRCA-associated ring domain-1) Polymorphism

Kim, Jin Hwi; Lee, Sung Jong; Lee, Keun Ho; Kim, Chan Joo; Namkoong, Sung Eun; Park, Jong Sup

Abstract

OBJECTIVE
The aim of this study was to identify interaction between causative factors of cervical cancer and BARD-1 (BRCA1 associated ring domain-1) polymorphism on cervical carcinogenesis in Korean women.
METHODS
We evaluated 1,347 patients composed of women who had cervical cancer (n=337) and cervical intraepithelial neoplasia (n=308) and normal control healthy women (n=702) from October 2000 until November 2001. The single nucleotide polymorphisms (SNPs) of BARD-1 were evaluated from extracted DNA of peripheral blood with automatic DNA sequencer. The difference of each SNP between normal controls and patients was evaluated in the adjusted environmental background.
RESULTS
In environmental aspect, the cervical cancer increased in the women who had the lower level of education, smoking history, the younger age of the first sexual intercourse, and the more child-bearing . The women who had BARD V507M (Arg/Arg) showed 1.6 fold increased risk in cervical cancer than the women who had BARD V507M (G/G), (G/A)(p=0.0433). And the risk increased with BARD R378S (C/C) among who have environmental risk factors compared with BARD R378S (G/G), (G/C) allele positive who have no environmental risk factors.
CONCLUSION
We found that the level of education, the age at first intercourse, and the number of children were the independent risk factors in cervical carcinogenesis. The specific combination of BARD-1 and gene-environmental interactions were significantly noted in the cervical carcinogenesis in Korean women.