Korean J Obstet Gynecol.  2003 Sep;46(9):1707-1713.

The Expression of c-Src and Csk in the Human Endometrium During the Normal Menstrual Cycle

• Jeong DK
• Lee SK
• Kim KJ
• Jang BW
• Han CH
• Lee JY
• Kim ST
• Kim YM

Abstract

OBJECTIVE
The purpose of this study was to investigate the transcriptional expression and translational localization of c-Src and Csk, related to signal transduction of cell growth, differentiation, death, and DNA synthesis in the human endometrium during the normal menstrual cycle.
METHODS
The specimens were obtained from the hysterectomized uterus and endometrial biopsies that showed no endometrial pathology. The mRNA level of c-Src and Csk were examined by RT-PCR and the expression of these proteins were confirmed by immunohistochemistry.
RESULTS
The c-Src mRNA was strongly expressed at all phases, except in late secretory phase. The Csk mRNA was highly expressed at all phases. In stromal cells and glandular cells, immunohistochemical expression of c-Src protein was moderate in proliferative phase and was strong in the secretory phase. The expression of Csk protein was strong at all phases and more intensive in glandular cell than stromal cell.
CONCLUSION
It is suggested that c-Src may play an important role in the signaling of the differentiation of the human endometrium and the Csk may modulate the c-Src kinase activity.

Keyword

c-Src; Csk; Endometrium; Menstrual cycle

MeSH Terms

Biopsy
DNA
Endometrium*
Female
Humans*
Immunohistochemistry
Menstrual Cycle*
Pathology
Phosphotransferases
RNA, Messenger
Signal Transduction
Stromal Cells
Uterus
DNA
Phosphotransferases
RNA, Messenger