Study of bony trabecular characteristics using bone morphometry and micro-CT

Song, Young Han; Lee, Wan; Lee, Chang Jin; Ji, Jung Hyun; Lee, Byung Do

Abstract

PURPOSE: The research was done to investigate the effectiveness of 2D bony morphometry and microstructure of micro-computed tomography (micro-CT) on the osteoporotic bony change. We performed the bone morphometric analysis of proximal femur in ovariectomized rabbits with BMD and micro-CT examination.
MATERIALS AND METHODS
Twenty-one female (Newzealand, about 16 weeks old, 2.9-3.4 kg) rabbits were used. Three rabbits were sacrificed on the day when experiment began (Baseline). The remaining 18 rabbits were divided into two groups. One group was ovariectomized bilaterally (OVX) and the other animals were subjected to sham operation (Sham). Bone specimens were obtained from the right and left femur of sacrificed rabbits. At intervals of 1, 2, 3, 5, 6 months respectively, BMD tests were performed on the proximal femur by using PIXImus 2 (GE Lunar Co. USA), 2-dimensional bone morphometric analysis by custome computer program and 2D / 3D bone structure analysis by micro-CT (Skyscan1072, Antwerpen, Belgium). Statistical analysis was carried out for the correlation between bone morphometry , micro-CT and BMD
RESULTS
BV/TV, Tb.Th, Tb.N of micro-CT parameters showed higher values in sham group than OVX group. N.Nd/Ar.RI, N.NdNd, N.NdTm, N. TmTm, PmB/Ar.RI, 3-D BoxSlope of 2D morphometric parameters showed higher values in Sham group than OVX group.The micro-CT parameters of Tb.Sp, Tb.N were statistically significant correlated with BMD respectively. Several 2D morphometric parameters were statistically significant correlated with BMD respectively.
CONCLUSION
Several parameters of 2D bony morphometry and micro-CT showed effective aspects on the osteoporotic bony change.