Korean J Obstet Gynecol.  2002 Dec;45(12):2130-2139.

Transforming Growth Factor-alpha Regulation of Insulin-like Growth Factor-II, Insulin-like Growth Factor Binding Protein-1 and 3 secretion and Epidermal Growth Factor receptor expression in human luteinized granulosa cells

• Kang EH
• Hong SH
• Cho JS
• Kim SH
• Chae HD
• Kim CH
• Kang BM
• Nam JH

Abstract

OBJECTIVE
To investigate the effects of transforming growth factor (TGF)-alpha on insulin-like growth factor (IGF)-II, insulin-like growth factor binding protein (IGFBP)-1, and 3 secretion and epidermal growth factor (EGF) receptor expression in cultured human luteinized granulosa cells.
MATERIALS AND METHODS
Human luteinized granulosa cells were obtained from follicular fluid by transvaginal oocyte aspiration from infertile patients undergoing in vitro fertilization and cultured for 72 hours with TGF-alpha at concentration of 1.0, 10.0, 100.0 ng/ml. The luteinized granulosa cells not treated with TGF-alpha served as control. The secretion of IGF-II, IGFBP-1 and 3 were determined in conditioned media by immunoradiometric assay (IRMA) and reverse transcription-polymerase chain reaction (RT-PCR) was performed for EGF receptor mRNA expression.
RESULTS
The cell numbers of 1.0 and 10.0 ng/ml supplement groups were significantly decreased compared to control (p<0.05, p<0.05, respectively), although the cell viabilities were similar in all groups. IGF-II levels were significantly higher in TGF-alpha treatment group at 1.0 and 10.0 ng/ml (p<0.01, p<0.01, respectively), but lower in 100.0 ng/ml (p<0.01). However, the concentrations of IGFBP-1, and 3 per one granulosa cell in each group were no statistically significant differences among the groups. The mRNA concentration of EGF receptor in cultured human luteinized granulosa cells were not significantly different among the groups.
CONCLUSION
This study suggests that TGF-alpha regulate intrafollicular bioavailable IGF-II levels, by which TGF-alpha might involved luteinizations. However, TGF-alpha may not directly regulate EGF receptor mRNA expression in cultured human luteinized granulosa cells.

Keyword

Transforming growth factor-alpha; Human luteinized granulosa cell; Insulin-like growth factor-II; Insulin-like growth factor binding protein-1; Insulin-like growth factor binding protein-3; Epidermal growth factor receptor

MeSH Terms

Carrier Proteins
Cell Count
Cell Survival
Culture Media, Conditioned
Epidermal Growth Factor*
Female
Fertilization in Vitro
Follicular Fluid
Granulosa Cells*
Humans*
Immunoradiometric Assay
Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor II
Lutein*
Oocyte Retrieval
Receptor, Epidermal Growth Factor*
RNA, Messenger
Transforming Growth Factor alpha
Transforming Growth Factors
Carrier Proteins
Culture Media, Conditioned
Epidermal Growth Factor
Insulin-Like Growth Factor Binding Protein 1
Insulin-Like Growth Factor II
Lutein
RNA, Messenger
Receptor, Epidermal Growth Factor
Transforming Growth Factor alpha
Transforming Growth Factors