Ann Dermatol.  2010 Nov;22(4):412-417. 10.5021/ad.2010.22.4.412.

Chemokine Receptor CCR3 Expression in Malignant Cutaneous Tumors

• Lee YJ
• Kim DH
• Lee SH
• Nam HS
• Roh MR
• Cho MK

• KMID: 2266177
• DOI: http://doi.org/10.5021/ad.2010.22.4.412

Abstract

BACKGROUND
Chemokines and their receptors are important players in tumorigenesis by facilitating tumor proliferation and metastasis. Little is known about the possible function of chemokine receptors in relation to the development and progression of malignant cutaneous tumors.
OBJECTIVE
The aim of this study was to determine the chemokine receptor CCR3 expression pattern and the protein expression level in selected malignant cutaneous tumors.
METHODS
Four types of cell lines (G361, A431, SK-MEL-2, SK-MEL-24) were analyzed, using Western blotting, for the expression of CCR3 protein. Immunohistochemical staining for CCR3 was done on 36 skin cancer tissue samples that included 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs), 16 malignant melanomas (MMs) and 6 normal tissue samples.
RESULTS
Western blot analysis showed that CCR3 protein was more expressed in the MM cell lines (G361, SK-MEL-2,SK-MEL-24) than that in the SCC cell line (A431), and the immunohistochemical analysis showed that CCR3 protein was overexpressed in MM and SCC, it was mildly expressed in BCC and it was hardly expressed in normal tissue.
CONCLUSION
This study demonstrated via immunochemistry that CCR3 was more expressed in MM, followed by SCC and BCC. The existence of CCR3 protein may enhance the tumorigenic potential of malignant cutaneous tumors.

Keyword

CC chemokine receptors; CCR3; Chemokine

MeSH Terms

Blotting, Western
Carcinoma, Basal Cell
Carcinoma, Squamous Cell
Cell Line
Cell Transformation, Neoplastic
Chemokines
Immunochemistry
Melanoma
Neoplasm Metastasis
Receptors, CCR
Receptors, Chemokine
Skin Neoplasms
Chemokines
Receptors, CCR
Receptors, Chemokine

Figure

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  Fig. 1 CC chemokine receptor CCR3 protein was expressed on squamous cell carcinoma cell line, malignant melanoma cell lines by western blot analysis.

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  Fig. 2 The score of relative CCR3 expression amount in western blotting.

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  Fig. 3 Representative immunohistochemistry staining for CCR3 protein expression in paraffin-embedded malignant melanoma (A: strongly positive immunostaining, ×200), squamous cell carcinoma (B: moderately positive immunostaining, ×200), basal cell carcinoma (C: weak positive immunostaining, ×200) and normal skin (D: no immunostaining, ×100).

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