Ann Dermatol.  2012 Aug;24(3):287-294. 10.5021/ad.2012.24.3.287.

Molecular Phylogenetics of Exophiala Species Isolated from Korea

• Suh MK
• Lee HC
• Kim DM
• Ha GY
• Choi JS

• KMID: 2265301
• DOI: http://doi.org/10.5021/ad.2012.24.3.287

Abstract

BACKGROUND
Recently, identification of fungi have been supplemented by molecular tools, such as ribosomal internal transcribed spacer (ITS) sequence analysis. According to these tools, morphological Exophiala species was newly introduced or redefined.
OBJECTIVE
This study was designed to investigate the phylogenetics based on ribosomal ITS sequence analysis from clinical Exophiala species isolated in Korea.
METHODS
The strains of Exophiala species were 4 clinical isolates of phaeohyphomycosis agents kept in the department of dermatology, Dongguk University Medical Center(DUMC), Gyeongju, Korea. The DNAs of total 5 strains of Exophiala species were extracted by bead-beating method. Polymerase chain reaction of ITS region using the primer pairs ITS1-ITS4, was done and phylogenetic tree contributed from sequences of ITS region from 5 Korean isolates including E. dermatitidis CBS 109154 and comparative related strains deposited in GenBank.
RESULTS
The strains of Exophiala species were 3 strains of E. dermatitidis, 1 strain of E. jeanselmei and 1 strain of Exophiala new species. Among the 3 subtypes (type A, B, C) of E. jeanselmei, E. jeanselmei DUMC 9901 belonged to type B. Of the 2 main types of E. dermatitidis (type A, B) and 3 subtypes of E. dermatitidis type A (A0, A1 and A2), two strains (E. dermatitidis CBS 709.95, E. dermatitidis CBS 109154) belonged to A0 subtypes, 1 strain (E. dermatitidis DUMC 9902) A1 subtype, respectively.
CONCLUSION
Phylogenetic analysis of ITS region sequence provided useful information not only for new species identification but for the subtyping and origin of Exophiala species.

Keyword

Exophiala; Phylogeny; Ribosomal internal transcribed spacer

MeSH Terms

Dermatology
DNA
Exophiala
Fungi
Korea
Phaeohyphomycosis
Phylogeny
Polymerase Chain Reaction
Sequence Analysis
Sprains and Strains
DNA

Figure

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  Fig. 1 Neighbor-joining tree based on sequences of the ITS region from the 25 members of Exophiala species and relatives; neighbor-joining algorithm with 1,000 bootstrap replicates. R. aquaspersa CBS 313.73 is taken as an outgroup. ITS: internal transcribed spacer, E.: Exophiala, R.: Rhinocladiella, sp.: species, DUMC: Dongguk University Medical Center.

• 

  Fig. 2 Neighbor-joining tree based on sequences of the ITS region from the 11 members of E. jeanselmei and relatives; neighbor-joining algorithm with 1,000 bootstrap replicates. E. spinifera CBS 899.68T is taken as an outgroup. ITS: internal transcribed spacer, E.: Exophiala.

• 

  Fig. 3 Neighbor-joining tree based on sequences of the ITS region from the 15 members of E. dermatitidis and relatives; neighbor-joining algorithm with 1,000 bootstrap relatives E. spinifera CBS 899.68T is taken as an outgroup. ITS: internal transcribed spacer, E.: Exophiala.

• 

  Fig. 4 Neighbor-joining tree based on sequences of the ITS region from the 9 members of Exophiala species DUMC 0501 (new species) and relatives; neighbor-joining algorithm with 1,000 bootstrap replicates. ITS: internal transcribed spacer, DUMC: Dongguk University Medical Center, E.: Exophiala, u.: uncultured, f.: fungal.

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