Korean J Obstet Gynecol.  2001 Aug;44(8):1442-1449.

Influence of prostaglandin E2 and prostacyclin on vascular endothelial growth factor and basic fibroblast growth factor expression in cultured human trophoblast

• Roh SR
• Kim DJ
• Ahn HY
• Kang MJ
• Lee BH
• Lee JH
• Oh MJ
• Shin JC
• Kim SP

Abstract

BACKGRUOND: Several angiogenic factors such as bFGF and VEGF have been shown angiogenesis of placenta. PGE2 and PGI2 may be important in successful establishment of pregnancy.
OBJECTIVE
We studied to investigate whether PGE2 and PGI2 regulate expression of VEGF and bFGF gene in the cultured human trophoblast cells.
METHODS
Human trophoblasts were isolated from the placenta of early gestation (6-12 weeks). Isolated trophoblasts were cultured in the different concentration of PGE2 and PGI2 and according to the different cultured time of PGE2 and PGI2, respectively. Total RNA was extracted and RT-PCR was performed. RESULT: Expression of bFGF was increased in 10-7M and 10-6M of PGE2 and was always increased in the all different concentration of PGI2. Four isoforms (VEGF121, VEGF165, VEGF189, VEGF206) were always expressed in the all different PGE2 and PGI2 concentration compared to the control group. However, there was no significant difference in the all different PGE2 and PGI2 concentration. In both PGE2 and PGI2 treatment group, expression of bFGF was decreased at 60 min compared to the control group and was gradually increased in time-dependent pattern. At 180 min, its expression was similar to the control group.
CONCLUSION
Our data suggest that the expression of bFGF gene is influenced by cultured time and concentration of PGE2 and PGI2, although the expression of VEGF gene is not influenced.

Keyword

cultured human trophoblast; VEGF; bFGF; PGE2; PGI2

MeSH Terms

Angiogenesis Inducing Agents
Dinoprostone*
Epoprostenol*
Fibroblast Growth Factor 2*
Humans*
Placenta
Pregnancy
Protein Isoforms
RNA
Trophoblasts*
Vascular Endothelial Growth Factor A*
Angiogenesis Inducing Agents
Dinoprostone
Epoprostenol
Fibroblast Growth Factor 2
Protein Isoforms
RNA
Vascular Endothelial Growth Factor A