Effects of 12-o-tetradecanoyl-phorbol-13-acetate on beta-hCG secretion by cultured peripheral mononuclear cells during pregnancy

Cha, Dong Hyun; Yoo, Seung Min; Min, Gyung Hee; Kim, Duk Ryung; Kim, Geun Ha; Sung, Yeon Joon; Kim, Dong Gyu; Lee, Byung Suk; Seo, Gyung; Lee, Yoon Ho; Lee, Gook

Abstract

OBJECTIVE
Peripheral blood mononuclear cells (PBMC) in culture release a biologically active human chorionic gonadotropin (hCG). This effect is detectable during pregnancy with a maximum between the 16th and 19th week. HCG plays an important role for the corpus luteum rescue during the early gestational age and possibly for the immunotolerance. This study was performed to investigate the relationships between the productivity of cultured PBMC of pregnant women and the ability to maintain early pregnancy, and whether 12-o-tetradecanoyl-phorbol-13-acetate (TPA) increases hCG sectetion by cultured PBMCs.
MATERIALS AND METHODS
PBMC were obtained from 20 pregnant women between 16th to 19th week of gestation , and cultured with TPA. Culture cells were harvested and hCG mRNA were extracted and RT-PCR were performed. Culture supernatants were collected and hCG concentration were determined by commercial RIA methods.
RESULTS
The mean age was 31.0 years old, 19 of 20 (95%) pregnant women's PBMC secereted hCG and expressed hCG mRNA, but in control group exept male hepatitis B patient, none of them produced hCG. TPA activated expression of hCG in PBMC in linear manner.
CONCLUSION
Pregnant women's cultured PBMC secreted hCG, but not in non-pregnant or male. We could confirm the mRNA of hCG in PBMC as well in the placental control. The productivity of hCG in PBMC might be closely related with maintenance of early pregnancy.