J Korean Neurosurg Soc.  1995 Jul;24(7):743-752.

Phorbol Esters Attenuate The Action of Isoproterenol on Vascular Smooth Muscle

• Kim BY
• Lee YW

Abstract

The effects of phorbol esters were studied in rabbit carotid artery to evaluate the action of protein kinase C on the regulation of vascular tone by isoproterenol. The vascular rings, 2 mm in width, were myographied isometrically in an isolated organ bath and the effects of phorbol 12,13-dibutyrate(PDBu) and phorbol 12-myristate 13-acetate(PMA) were determined. Isoproterenol, a beta adrenergic agonist, relaxed the vessel which was precontracted by phenylephrine, but not that by phorbol esters. The action of isoproterenol was attenuated by removal of endothelium or pretreatment with methylene blue or nitro-L-arginine. The pretreatment with phorbol esters at concentrations which did not induce contraction, decreased isoproterenol-induced relaxation of vascular rings with or without endothelium. The action of PDBu on isoproterenol-induced relaxation was less effective than that of PMA, unlike those observed in contractile response, but the contractile effect of the former was more potent than that of the latter. PMA did not affect relaxant effect of forskolin, an activator of adenyl cyclase. Staurosporine, a protein kinase C inhibitor, inhibited the action of these drugs on both isoproterenol-induced relaxation and the contractile response. These results suggest that the relaxation induced by isoproterenol was reducd by the activation of protein kinase C, which may be isozyme different from that involved in contractile response.

Keyword

Phorbol esters; Protein kinase C; Isoproterenol; Beta adrenergic receptor; Rabbit carotid artery

MeSH Terms

Adenylyl Cyclases
Adrenergic beta-Agonists
Baths
Carotid Arteries
Colforsin
Endothelium
Isoproterenol*
Methylene Blue
Muscle, Smooth, Vascular*
Phenylephrine
Phorbol Esters*
Protein Kinase C
Relaxation
Staurosporine
Adrenergic beta-Agonists
Colforsin
Isoproterenol
Methylene Blue
Phenylephrine
Phorbol Esters
Protein Kinase C
Staurosporine