Korean J Nephrol.  1997 Jun;16(2):221-229.

Culture of the Human Glomerular Endothelial Cells

• Park SK
• Ahn HJ
• Kim SW
• Lee JD
• Park JS

Abstract

Whereas mesangial and epithelial cells from glomeruli are commonly grown in vitro, there has been major difficulties in developing homogenous cultures of human glomerular endothelial cells. This study defines the conditions for the reproducible isolation and growth of homogenous monolayers of human glomerular endothelial cells based on the method of Green DF et al published in 1992. Using the selective media and the sieving method, fibronectin was required as a surface matrix after adequate collagenase treatment, and endothelial cell growth factor and heparin was needed for the continuous growth of endothelial cells. The endothelial cell growth factor was isolated from the bovine hypothalamic extracts. Glomerular capillary endothelial cells exhibited a cobblestone morphology at confluence and stained homogenously with von Willebrand factor(factor VIII). The cytokeratin and the actin were not stained. This study might be helpful for in vitro study to know the biological characteristics of human glomerular endothelial cells under the predetermined condition.

Keyword

Cell culture; Human glomerular endothelial cells; Endothelial cell growth factor(ECGF)

MeSH Terms

Actins
Cell Culture Techniques
Collagenases
Endothelial Cells*
Epithelial Cells
Fibronectins
Heparin
Humans*
Keratins
Population Characteristics
Actins
Collagenases
Fibronectins
Heparin
Keratins