Korean J Hematol.  2009 Sep;44(3):127-132. 10.5045/kjh.2009.44.3.127.

Allele-related Variation in Minisatellite Repeats Involved in Transcription of the ABO Gene in Korean Blood Donors

• Kim SH
• Cho D
• Kee SJ
• Shin MG
• Shin JH
• Suh SP
• Ryang DW

• KMID: 2252129
• DOI: http://doi.org/10.5045/kjh.2009.44.3.127

Abstract

BACKGROUND: The CBF/NF-Y enhancer region of ABO gene reported to contain 43bp minisatellite tandem repeats has been rarely reported. We describe here the relationship between minisatellite tandem repeats and ABO alleles in samples from Korean population with common ABO blood group and rare ABO subgroup.
METHODS
Sixty one cases of ABO subgroup (14 A2, 12 A2B, 1 Aweak, 7 AweakB, 11 B3, 5 A1B3, 1 A1Bweak, 2 Bweak, and 8 cis-AB) and 41 cases of common ABO blood group (13 A, 6 AB, 11 B, and 11 O) were obtained from healthy donors at the Gwangju-Chonnam Red Cross Blood Center between Sep 2004 and Aug 2005. Red cells were phenotyped by standard serologic tests and genotyped by direct DNA sequencing exon 6 and 7 of the ABO gene. The minisatellite repeats were analyzed by PCR method.
RESULTS
The ABO*A101 and *A102 had only one repeat, *B101, *O01 and *O02 had 4 repeats in common ABO blood group, while the *A102, *cis-AB01, and *Aw10 had only one minisatellite repeat and *A201, *A204, *B101, *Bw03, *B306, *O01, and *O02 alleles had 4 repeats and unexpectedly 3 A2 cases with *A102 had 4 repeats in the rare ABO subgroup.
CONCLUSION
The minisatellite repeats found in Koreans correlate well with ABO alleles in sample common ABO phenotype, but do not completely correlate with those of ABO subgroup. We revealed here a pattern of the minisatellite repeats in various ABO subgroup in Korea.

Keyword

ABO genotype; ABO subgroups; Direct DNA sequencing; Minisatellite repeats; Enhancer

MeSH Terms

Alleles
Blood Donors
Exons
Humans
Korea
Minisatellite Repeats
Phenotype
Polymerase Chain Reaction
Red Cross
Sequence Analysis, DNA
Serologic Tests
Tandem Repeat Sequences
Tissue Donors

Figure

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  Fig. 1. (A) Structure of the CBF/ NF-Y enhancer region (rectangle with shading patterns) of ABO genes and primers Pro-F (5'GG AAACAAATCCTACCCCTAC3') and Pro-R (5'GTGCTGCCTGTGC CTGTTAC3') used in this study. (B) PCR products from representative ABO subgroups and their genotypes separated in 1.8% agarose gel after PCR amplification with primers Pro-F and Pro-R. Lane, M. molecular size marker; 1. A2 (A101/O02); 2. A2 (A201/O01); 3. A2B (A102/B101); 4. A2B (A204/B101); 5. AwB (Aw10/B101); 6. B3 (B101/O01); 7. A1B3 (A102/B101); 8. A1B3 (cis-AB01/A102); 9. A2B (A102/ B306); 10. A2B3 (cis-AB01/B101); 11. B3 (cis-AB01/O01).

Reference

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