Korean J Lab Med.  2005 Aug;25(4):280-284.

Molecular Characteristics of B Subgroups in Koreans

Affiliations
  • 1Department of Blood Service Headquarters and Central Blood Laboratory Center, Korea.
  • 2Department of The Republic of Korea National Red Cross, Korea.
  • 3Department of Seoul National University Hospital Clinical Research Institute, Seoul National University College of Medicine, Seoul, Korea. kshanmd@snu.ac.kr
  • 4Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
  • 5Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
An accurate ABO blood type is essential for transfusion therapy. Genetic structures of ABO blood group and subgroup have been investigated and so far about 100 ABO alleles have been reported worldwide. This study was performed to investigate the molecular characteristics of B subgroups in the Korean population. METHODS: A total of 19 samples of B subgroups were collected from patients (n=11) and from blood donors (n=8) of Korean Red Cross blood centers; these samples had been typed serologically for the ABO blood group. Allele-specific polymerase chain reaction (PCR), direct sequencing of exon 6 and 7, and allele separation were performed for ABO gene analysis.
RESULTS
The ABO PCR-RFLP genotyping results of 18 samples among the provisional 19 B subgroups were identical regardless of their phenotypes. Two new B alleles showing 255C>T base change and 547G>A base change were observed in B3 and A1B3 subgroups.
CONCLUSIONS
Serologically unidentified B subgroups were unequivocally identified through molecular analyses of the ABO gene. And new ABO alleles observed only in the Korean B subgroups were recognized.

Keyword

B subgroup; ABO gene; Allele; Polymorphism; Koreans

MeSH Terms

Alleles
Asian Continental Ancestry Group
Blood Donors
Exons
Genetic Structures
Humans
Phenotype
Polymerase Chain Reaction
Red Cross
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