Korean J Lab Med.  2004 Feb;24(1):60-66.

Development of PCR-ELISA for Detection of HIV-1 in Biomedical Products

• Shim JH
• Kim JW
• Lee KA
• Kwon DH
• Myung PK
• Kim JO
• Lee SH
• Park SN
• Yoon DY

Abstract

BACKGROUND
Biomedical products such as viral vaccines can be contaminated with hazardous viruses during manufacturing processes and storage, thus causing harmful side effects. To assure the safety of biomedical products, highly effective and sensitive methods should be available to detect contaminating viruses. In this study, we performed recovery tests to determine the limit of detection of HIV-1. METHODS: An HIV-1 plasmid preparation was serially diluted and spiked into various culture media (DMEM, RPMI-1640, IMDM, GICM, and SDM) containing 10% fetal bovine serum (FBS). The HIV-1 plasmid was detected by PCR alone or a combination of PCR and ELISA (PCR/ELISA). RESULTS: When spiked into DMEM, RPMI, and IMDM, less than 4x10(-2) ng of HIV-1 plasmid was not detectable as HIV-1 PCR products in agarose gel. Intra- and inter-assays (n=6) showed that the PCR-ELISA system could detect PCR products diluted as much as 1, 875 times from HIV-1 plasmid serially spiked in various media. CONCLUSIONS: The PCR/ELISA system can be useful for the detection of trace amounts of hazardous viruses which may be present as contaminants in biological products.

Keyword

HIV-1; PCR-ELISA

MeSH Terms

Biological Products
Culture Media
Enzyme-Linked Immunosorbent Assay
HIV-1*
Limit of Detection
Plasmids
Polymerase Chain Reaction
Sepharose
Viral Vaccines
Biological Products
Culture Media
Sepharose
Viral Vaccines