Korean J Lab Med.  2006 Aug;26(4):246-248. 10.3343/kjlm.2006.26.4.246.

Chronic Myelogenous Leukemia with a Variant Philadelphia Translocation: t(11;22)(q25;q11.2)

• Kim HS
• Cho HC
• Kim SH
• Moon Y
• Nahm CH
• Choi JW
• Kim JJ

• KMID: 2238830
• DOI: http://doi.org/10.3343/kjlm.2006.26.4.246

Abstract

We report a case of chronic myelogenous leukemia displaying a variant Philadelphia translocation t(11;22)(q25;q11.2). Breakpoint 11q25 has not previously been reported. Reverse transcriptase polymerase chain reaction and fluorescence in-situ hybridization demonstrated the BCR/ABL rearrangement.

Keyword

Variant Philadelphia; t(11;22)(q25;q11.2); Chronic myelogenous leukemia

MeSH Terms

Fluorescence
Leukemia, Myelogenous, Chronic, BCR-ABL Positive*
Reverse Transcriptase Polymerase Chain Reaction

Figure

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  Fig. 1. The G-banded karyotype of the patient was determined as 46,XY,t(11;22)(q25;q11.2).

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  Fig. 2. LSI BCR/ABL dual color, dual fusion probe (cited by http://www.vysis.com/AnalyticSpecificReagents(ASR)_59424.asp?PrintPage=true&ProdID=35–191032, Vysis, Downers Grove, IL, USA.). The BCR probe was labeled with a green fluorochrome (G) and the ASS-ABL probe was labeled with a orange fluorochrome (O). BCR/ABL rearrangements were detected as yellow fusion signals (F). The 2O2G was normal signal pattern. The 1O1G2F was typical pattern for BCR/ABL fusion. Atypical patterns of 1O1G1F, 1O2G1F and 2O1G1F are indicative of genomic deletions[9, 11].

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  Fig. 3. D-FISH for BCR/ABL. (A) Two orange signals, a green signal, and a fusion signal in an interphase. (B) Two orange signals on chromosome 9, a green signal on chromosome 22, and a fusion signals (arrow) in a metaphase.

Reference

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