Effect of Muscle Relaxation by Domestic Product of Metuben in Rabbit

Chun, Yong Suk; Kim, Dong Hwan; Jun, Jong Hun; Shim, Jae Chul; Kim, Kyoung Hun; Lee, Dong Ho; Kim, Kyo Sang; Suh, Jung Kook; Yoo, Hee Koo; Chon, Se Ung

Abstract

The domestic product of a non-depolarizing muscle relaxant, Metuben, was studied on its effect in rabbit. Twenty four rabbits of either sex weighing 2~3kg were divided into four groups (consisting of 6 animals each) to administer Metuben in doses of 0.05 mg/kg, O.l mg/kg, 0.2 mg/ kg and 0.4 mg/kg, respectively. All animals were anesthetized with 20% urethane 5 ml/kg given intraperitoneally and 2.5% pentothal sodium 10 mg/kg intravenously. ECG was monitored by Physio-contro1(lifepak 7)model. The animal lungs were mechanically ventilated through a tracheostomy and Shinano animal respirator set to deliver a 30 ml/kg tidal volume at 30 breaths/min. This ventilatory pattern resulted in the PaCO2 values within the range of 30-40 mmHg. Twitches of the tibialis anterior muscle were elicited at 0.1 Hz, "Train of four" via the peroneal branch of the sciatic nerve, and the Myotest stimulator were applied at supramaximal voltage. Twitch recording was done via Biophysiograph. Results were as follows: 1) The effect of Metuben as a dose-dependent long lasting non-depolarizing muscle relaxant was confirmed. Their relaxant effect in rabbits were quite different from man. 2) In rabbits, Metuben showed great individual differences in its effect even with the s'ame dosage. The length of muscle relaxing activity.and the occurence of recurarization after neostigmine reverse were extremely variable suggesting its safety margin is quite narrow.