Korean J Fertil Steril.  2000 Mar;27(1):31-38.

Establishment of In Vitro 3-Dimensional Culture System of Mouse Endometrial Cells I. Cytohistological Study on Mouse Endometrium

• Nam HK
• Kim EY
• Lee KS
• Park SY
• Park EM
• Park SP
• Kwon JK

Abstract

This study was designed to identify the ultrastructural changes of mouse endometrum during peri-implantation period and obtain the fundamental information for the establishment of 3-dimensional culture system of mouse endometrial cells in vitro. The used female ICR mice (6~8 wks) were conducted on pregnant. The biopsies were obtained from whole uterus at cycle day 1 (D1) and day 5 (D5) after hCG injection and mating. The biopsies materials were fixed 2.5% glutaraldehyde and 1% osmium tetroxide. Subsequently, for observation using light and transmission electron microscopy (LM and TEM), they were dehydrated and embedded in Epon and the embedded biopsies were sectioned and stained. For scanning electron microscypy (SEM), the fixed specimens were dehydrated, dried and coated with gold. 1)For LM, the biopsied materials at D5 (late secretory phase) were appeared the extended stromal layer by increased connective tissues and the fully developed endometrial glands and vessels compared with D1 (early secretory phase). 2) For TEM, the mouse endometrium was consisted of 3-layers, a simple polarized columnar epithelial cells, basement membrane and stromal cells. At D5, the distribution of microvilli, endoplasmic reticulum, Golgi body, lipid and glycogen deposits, secretory granules and surface area of basement membrane were increased. 3) For SEM, the degree of folding and microvilli of surface of mouse epithelial cells was became more and more according to the process of secretory phase, and at D5, implantation time of mouse, the appearance of pinopodes as a specific marker of uterine receptivity was found. The uterine pinopodes of mouse were found in narrow sites at the luminal surface, irregularity and appeared the different stages in the same sample. Therefore, these results indicated that the mouse endometrium was experienced dramatic morphological changes during peri-implantation period.

MeSH Terms

Animals
Basement Membrane
Biopsy
Connective Tissue
Endometrium*
Endoplasmic Reticulum
Epithelial Cells
Female
Glutaral
Glycogen
Humans
Mice*
Mice, Inbred ICR
Microscopy, Electron, Transmission
Microvilli
Osmium Tetroxide
Phenobarbital
Secretory Vesicles
Stromal Cells
Uterus
Glutaral
Glycogen
Osmium Tetroxide
Phenobarbital