The Phenotypic Changes in B16F1 Mouse Melanoma Cells According to Various Media

Lee, Mu Hyoung; Jun, Il Sun

Abstract

BACKGROUND: The influencion the environment on a culture is expressed via four routes. (1) the nature of the substrate or phase on or in which the cells grow (2) the physicochemical and physiological constitution of the medium, (3) the constitution of the gas phase, and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium.
OBJECTIVE
The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B,F, mouse melanoma cells according to different culture media.
METHODS
We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin conlensin BgF mouse melanoma cells cultured in Dulbeccos modified Eagles medium(DMEM), F-10, MCDB 153, Minimal essential medium(MEM), and RPMI 1640, respectively.
RESULTS
1. The color of cell pellet., ringed from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium. 2. Most Bg, mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media. 3. In the electron microscopic. findings, BF, mouse melanoma cells in DMEM and MEM con tained numerous stage IV nelanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few. 4. The number of BF, mouse melanoma cells were 1.42 + 0.06 x 10", 1.42 + 0.12 x 10", l. 17 + 0.08 x 10, 0.73 0.06 x 10, 0.32 0.01 x 10, in RPMI 1640, DMEM, MEM, F 10, and MCDB 153 medium, respectively. 5. In the MTT assay, the order of the optical density of B,F, mouse melanoma cells in various media was as followings, DMEM, RPMI 1640, MEM, F-10, and MCDB 153. 6. Compared with the melanin contents of B;F, mouse melanoma cells in DMEM, they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.94% in F-10 medium.
CONCLUSION
The phenotypic changes of BF, mouse melanoma cells were induced by various culture rnedia and were reversilvle. Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for the cells.