Korean Circ J.  1997 Apr;27(4):433-437. 10.4070/kcj.1997.27.4.433.

Effect of Glutathione on Oxidant-induced Cardiotoxicity

• Yoon HS
• Park ST
• Park OK

• KMID: 2229058
• DOI: http://doi.org/10.4070/kcj.1997.27.4.433

Abstract

BACKGROUND
In order to elucidate toxic mechanism of the oxygen radicals on cultured rat myocardial cells, cytotoxic effect of oxygen radicals was evaluated by MTT assay. In addition protective effect of glutathione(GSH) on oxidant-induced cardiotoxicity was investigated on these cultures.
METHODS
Myocardial cells derived from neonatal rats were cultured for 12 hours in the medium containing various concentrations of glucose oxidase(GO). Cell viability was measured by MTT assay and morphological changes of the myocardial cells were observed by light microscope.
RESULTS
GO-mediated oxygen radicals remarkably decreased cell viability of cultured myocardial cells in a dose-and time-dependent manner. And also, GSH blicked GO-induced cardiotoxicity in these cultures.
CONCLUSION
These results suggest that the oxygen radicals are tixic and the selective antioxidants such as GSH are effective in blocking against the oxidant-induced cardiotoxicity in cultures of the myocardial cells of neonatsl rats.

Keyword

Cultured myocardial cells; Oxygen radicals; Cardiotoxicity; Antioxidant

MeSH Terms

Animals
Antioxidants
Cell Survival
Glucose
Glutathione*
Rats
Reactive Oxygen Species
Antioxidants
Glucose
Glutathione
Reactive Oxygen Species