Korean J Anesthesiol.  2014 Aug;67(2):123-128. 10.4097/kjae.2014.67.2.123.

Antiallodynic effect of intrathecal epigallocatechin-3-gallate due to suppression of reactive oxygen species

Affiliations
  • 1Department of Anesthesiology and Pain Medicine, Gwangju Christian Hospital, Gwangju, Korea.
  • 2Department of Anesthesiology and Pain Medicine, Chonnam National University, Medical School, Gwangju, Korea. mhyoon@chonnam.ac.kr
  • 3Center for Creative Biomedical Scientists at Chonnam National University, Gwangju, Korea.

Abstract

BACKGROUND
Green tea modulates neuropathic pain. Reactive oxygen species (ROS) are suggested as a key molecule in the underlying mechanism of neuropathic pain in the spinal cord. We examined the effect of epigallocatechin-3-gallate (EGCG), the major catechin in green tea, in neuropathic pain and clarified the involvement of ROS on the activity of EGCG.
METHODS
Neuropathic pain was induced in male Sprague-Dawley rats by spinal nerve ligation (SNL). A polyethylene tube was intrathecally located. Nociceptive degree was estimated by a von Frey filament and expressed as a paw withdrawal threshold (PWT). To determine the role of ROS on the effect of EGCG, a free radical donor (tert-BuOOH) was pretreated before administration of EGCG. ROS activity was assayed by xanthine oxidase (XO) and malondialdehyde (MDA).
RESULTS
SNL decreased the PWT compared to sham rats. The decrease remained during the entire observation period. Intrathecal EGCG increased the PWT at the SNL site. Intrathecal tert-BuOOH significantly decreased the effect of EGCG. The levels of both XO and MDA in the spinal cord were increased in SNL rats compared to sham. Intrathecal EGCG decreased the level of XO and MDA.
CONCLUSIONS
EGCG may reduce neuropathic pain by SNL due to the suppression of ROS in the spinal cord.

Keyword

EGCG; Neuropathic pain; ROS; Spinal cord

MeSH Terms

Animals
Catechin
Humans
Ligation
Male
Malondialdehyde
Neuralgia
Polyethylene
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species*
Spinal Cord
Spinal Nerves
Tea
Tissue Donors
Xanthine Oxidase
Catechin
Malondialdehyde
Polyethylene
Reactive Oxygen Species
Tea
Xanthine Oxidase
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