Korean Circ J.
2004 Mar;34(3):288-295. 10.4070/kcj.2004.34.3.288.
Serological Kinetics of Adenylate Kinase 3 in Rat Acute Myocardial Infarction
- Affiliations
-
- 1Division of Molecular Immunology, Department of Biochemistry & Molecular Biology, College of Science and Technology, Korea.
- 2Department of Thoracic Surgery, College of Medicine, Hanyang University, Seoul, Korea.
- 3Division of Cardiology, Department of Internal Medicine, College of Medicine, Hanyang University, Seoul, Korea.
- KMID: 2227215
- DOI: http://doi.org/10.4070/kcj.2004.34.3.288
Abstract
- BACKGROUND AND OBJECTIVES
Currently used serological markers for the diagnosis of acute myocardial infarction differ in appearance time and specificity for myocardial infarction, allowing no ideal single serological marker for myocardial infarction. Adenylate kinase (AK) is a ubiquitous enzyme which contributes to the homeostasis of the cellular adenine nucleotides pool. AK is abundant in the myocardium, and we postulated that AK3 could be used as a biochemical marker for the diagnosis of acute myocardial infarction (AMI). MATERALS AND METHODS: We constructed an AMI rat model with ligation of the anterior descending artery. We measured the concentration of serum AK3 in the AMI rat model by enhanced chemiluminescence (ECL) sandwich ELISA using monoclonal antibodies against recombinant AK3.
RESULTS
The serum AK3 level started to increase in 3 hours and reached a peak at 6 hours after ligation of the rat coronary artery. The significant elevation of AK3 was retained for 2 days (p<0.05).
CONCLUSION
AK3 is a useful serological marker for acute myocardial infarction in the rat.
MeSH Terms
-
Adenine Nucleotides
Adenylate Kinase*
Animals
Antibodies, Monoclonal
Arteries
Biomarkers
Coronary Vessels
Diagnosis
Enzyme-Linked Immunosorbent Assay
Homeostasis
Kinetics*
Ligation
Luminescence
Models, Animal
Myocardial Infarction*
Myocardium
Rats*
Sensitivity and Specificity
Adenine Nucleotides
Adenylate Kinase
Antibodies, Monoclonal
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