Korean J Cytopathol.  1996 Jun;7(1):38-43.

Application of Epstein-Barr Virus Cell Lines (CCL85 EB-3) in Performing the EBER mRNA In Situ Hybridization as a Positive Control

• Kim SS
• Han WS
• Suh JY
• Huh JR

Abstract

Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkin's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studies about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-embedded tissue. We applied Burkitts lymphoma cell line as positive control in EBV in hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

Keyword

Epstein-Barr virus; In Situ hybridization; EBER mRNA; Burkitt lymphoma

MeSH Terms

Biology
Burkitt Lymphoma
Carcinoma
Cell Line*
Central Nervous System
Herpesvirus 4, Human*
Hodgkin Disease
In Situ Hybridization*
Leukoplakia
Lymphoma
Lymphoma, T-Cell, Peripheral
RNA, Messenger*
RNA, Messenger