Korean J Clin Pathol.
1999 Dec;19(6):735-740.
Isolation of Candidate Tumor Suppressor Gene in Renal Cell Carcinoma
- Affiliations
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- 1Department of Biochemistry, Dong-A University College of Medicine, Pusan, Korea. jipark@seunghak.donga.ac.kr
- 2Department of Pathology, Dong-A University College of Medicine, Pusan, Korea
- 3Department of Clinical Pathology, Dong-A University College of Medicine, Pusan, Korea
- 4Department of Urology, Dong-A University College of Medicine, Pusan, Korea
Abstract
- BACKGROUND
Although renal cell carcinoma (RCC) is the most common malignancy originated from kidney in adults, pathogenesis of RCC remains unknown. The purpose of this work is to find tumor suppressor gene in RCC.
METHODS
The arbitrarily primed polymerase chain reaction (AP-PCR) has been used to detect somatic genetic alterations in RCC. DNA fingerprints generated by single arbitrary primers were compared between normal and tumor tissues of the same individuals. AP-PCR bands showing decreased intensities in tumor tissue DNA, relative to normal, have been cloned after reamplification with the same arbitrary primer. We have performed Southern blot hybridization and DNA sequencing.
RESULTS
For a given primer, at least 5 differences in band patterns between normal and tumor tissues were observed and band C was deleted in tumor tissues of clear cell type RCC. We found this band was split into 3 bands. Because band C2 was consistantly deleted in tumor tissue, we decided to clone and characterize this fragment. Partial DNA sequences of this fragment showed no homology with other genes by BLAST search. Southern blot analysis showed this fragment was deleted in 2 cases of clear cell type and 1 case of mixed cell type RCC.
CONCLUSIONS
These results suggest that fragment C2 might be a candidate for novel tumor suppressor gene and loss of this fragment might be necessary for malignant development to clear cell type RCC. Further characterization of this fragment is expected to give us useful informations about RCC tumorigenesis.
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