Korean J Clin Microbiol.
2006 Oct;9(2):102-109.
Emergence of CTX-M-12, PER-1 and OXA-30 beta-Lactamase-Producing Klebsiella pneumoniae
- Affiliations
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- 1Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea. kscpjsh@ns.kosinmed.or.kr
- 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Laboratory Medicine, Keonyang University College of Medicine, Daejeon, Korea.
- 4Department of Laboratory Medicine, Pochon Cha University College of Medicine, Sungnam, Korea.
- 5Department of Laboratory Medicine, Seoul National University College of Medicine, Korea.
- 6Department of Laboratory Medicine, The Catholic University College of Medicine, Korea.
- 7Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea.
- 8Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
- 9Department of Laboratory Medicine, Chonnam National University College of Medicine, Gwangju, Korea.
- 10Department of Laboratory Medicine, Ajou University College of Medicine, Suwon, Korea.
- 11Department of Laboratory Medicine, Sooncheonhyang University College of Medicine, Gumi, Korea.
- 12Department of Laboratory Medicine, Cheju Hanmaeum Hospital, Cheju, Korea.
- 13Center for Food Safety Evaluation, Korea Food and Drug Administration, Seoul, Korea.
Abstract
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BACKGROUND: The aim of this study was to determine a nation-wide prevalence of Ambler class A and D extended-spectrum-lactamases (ESBL) in Klebsiella pneumoniae isolates in Korea.
METHODS
During the period of April to May 2005, 189 isolates of K.pneumoniae were collected from 11 Korean hospitals. Antimicrobial susceptibilities to ceftazidime and cefotaxime were tested by the disk diffusion method, and ESBL production was determined by double-disk synergy test. Determinants of ceftazidime or cefotaxime-resistance were transferred to Escherichia coli J53 (azide-resistant) by transconjugation. Genotypes of class A and D ESBL genes were determined by PCR amplification and sequencing.
RESULTS
One hundred-sixty isolates of K.pneumoniae showed positive results in double-disk synergy test. The most prevalent ESBL was SHV-12 (n=148). Also detected were genes encoding ESBLs including TEM-52 (n=1), SHV-2a (n=2), CTX-M-3 (n=15), CTX-M-9 (n=6), CTX-M-12 (n=2), CTX-M-14 (n=9), CTX-M-15 (n=1), PER-1 (n=1), GES-5 (n=3), and OXA-30 (n=2) beta-lactamases.
CONCLUSION
With the emergence of CTX-M-12, PER-1, and OXA-30 beta-lactamases, the ESBLs in K.pneumoniae isolates are becoming more diverse in Korea.
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