Korean J Clin Microbiol.  2006 Oct;9(2):96-101.

Detection of Cytomegalovirus by Dual-PCR

• Choe WH
• Kang JO
• Choi TY
• Ahn Y

Abstract

BACKGROUND: Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality in transplant recipients and immunocompromised patients. We compared the results of a dual polymerase chain reaction (dual-PCR) and an antigenemia (Ag) test for detection of CMV from blood samples.
METHODS
Between February 2002 and May 2005, we analyzed 175 blood samples submitted for CMV tests at Hanyang University Hospital. The late antigen (LA) and major immediate early (MIE) genes of CMV were concurrently amplified in the dual-PCR. The lower matrix protein pp65 of CMV was detected for the Ag test (Chemicon, Temecula, CA, USA).
RESULTS
The positive rate of the dual-PCR was 14.3% (25/175) and that of the Ag test was 13.1% (23/175). The concordance rate of the dual-PCR and Ag test was 85.1% (149/175), while the discordance rate was 14.9% (26/175).
CONCLUSION
The dual-PCR is a useful method for the early detection of CMV, but we recommend using both the dual-PCR and Ag test for detection of CMV due to a high discordance rate of the two methods.

Keyword

Cytomegalovirus; Late antigen (LA) gene; Major Immediate Early Antigen (MIE) gene; Polymerase chain reaction; Antigenemia

MeSH Terms

Cytomegalovirus*
Immunocompromised Host
Mortality
Polymerase Chain Reaction
Transplantation