Ann Clin Microbiol.  2013 Mar;16(1):19-24. 10.5145/ACM.2013.16.1.19.

Comparison Cytomegalovirus Qualitative Assay Using Real-Time PCR and Conventional PCR

• Jeong S
• Kim YJ
• Bae IK
• Kim MJ
• Jeong SH

• KMID: 2169598
• DOI: http://doi.org/10.5145/ACM.2013.16.1.19

Abstract

BACKGROUND
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality in immunocompromised patients. We compared the abilities of the recently developed Real-Q Cytomegalovirus Kit (Biosewoom Inc., Korea) and the previously used PANA mPCR CMV Detection Kit (Panagene Inc., Korea) to detect CMV.
METHODS
We analyzed 300 samples (whole blood: 262, urine: 37, CSF: 1) submitted for qualitative CMV PCR testing during October 2011 at Yonsei University College of Medicine Severance Hospital. Real-time PCR was performed with a Real-Q Cytomegalovirus Kit and conventional PCR was conducted with a PANAmPCR CMV Detection Kit.
RESULTS
The positive rates of both Real-time PCR and conventional PCR were 25.3% (76/300), and the kappa coefficient (K) was 0.96 (95% confidence interval (CI), 0.93-1.00). The concordance rate of the Real-Q Cytomegalovirus Kit and the PANAmPCR CMV Detection Kit was 98.7% (296/300), and four out of 300 samples showed discordant results. If the concordant results of 296 samples and the four results confirmed by direct sequencing were assumed to be true, the sensitivity and specificity of the Real-Q Cytomegalovirus Kit were 97.4% (95% CI, 93.8-100.0%) and 99.1% (95% CI, 97.9-100.0%), respectively.
CONCLUSION
The recently developed Real-Q Cytomegalovirus Kit showed excellent sensitivity and specificity, and had a high concordance rate with the previously established PANAmPCR CMV Detection Kit, which uses conventional PCR. Furthermore, Real-time PCR could decrease the test time, as the electrophoresis step required for conventional PCR is not required for Real-time PCR.

Keyword

Cytomegalovirus (CMV); Polymerase chain reaction (PCR); Real-time polymerase chain reaction

MeSH Terms

Cytomegalovirus
Electrophoresis
Immunocompromised Host
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction

Figure

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  Fig. 1. Time for real-time PCR and conventional PCR per 12 samples.

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