J Korean Ophthalmol Soc.  2012 Dec;53(12):1864-1869. 10.3341/jkos.2012.53.12.1864.

Effect of Triamcinolone on Retinal Vessel-Related Factors in Oxygen-Induced Retinopathy Rats

• Yoo WS
• Seo SW
• Park JM
• Yoo JM
• Chung IY

• KMID: 2216403
• DOI: http://doi.org/10.3341/jkos.2012.53.12.1864

Abstract

PURPOSE
The present study investigated the effects of triamcinolone acetonide (TA) on retinal vessel-related factors and retinal vascular leakage in the retina of oxygen-induced retinopathy (OIR) rats.
METHODS
Sprague-Dawley rats used for OIR were exposed to hyperoxia from postnatal day 2 to day 14, then returned to normoxia from day 15 to day 30 and compared with control rats. On postnatal day 16, 2 microl of TA (4 mg/kg) was injected into the vitreous of the right eye in control and OIR rats. The Evans blue method was used for evaluating vascular leakage and RT-PCR was performed for confirmation of mRNA expression.
RESULTS
In OIR rats exposed to hyperoxia, retinal vascular permeability increased when returned to normoxia. After intravitreal injection of TA, vascular permeability was decreased in OIR rats, but no changes were found in control rats. In OIR rats, mRNAs of HIF-1alpha, VEGF, SDF-1 and ICAM-1 were more expressed and down-regulated after intravitreal injection of TA. Occludin mRNA level was decreased in the hypoxic condition and up-regulated after TA treatment.
CONCLUSIONS
The present study suggests the ability of TA to inhibit retinal vascular leakage in OIR rats and a possibility that TA controls expression of the HIF-1, VEGF, SDF-1, ICAM-1 and Occludin, which may protect retinal vascular destruction from hypoxic conditions; further studies are necessary to confirm changes in protein levels.

Keyword

Hypoxia inducible factor; Oxygen-induced retinopathy (OIR); Triamcinolone acetonide; Vascular endothelial growth factor

MeSH Terms

Animals
Capillary Permeability
Evans Blue
Eye
Hyperoxia
Intercellular Adhesion Molecule-1
Intravitreal Injections
Occludin
Rats
Rats, Sprague-Dawley
Retina
Retinaldehyde
RNA, Messenger
Triamcinolone
Triamcinolone Acetonide
Vascular Endothelial Growth Factor A
Evans Blue
Intercellular Adhesion Molecule-1
Occludin
RNA, Messenger
Retinaldehyde
Triamcinolone
Triamcinolone Acetonide
Vascular Endothelial Growth Factor A

Figure

• 

  Figure 1 Time course of vascular permeability changes in the retina of OIR rats. Vascular permeability in the retina was measured at P14, P16, P18, P20, P22, P26 and P30. The Evans blue in the tissues was normalized by total protein concentration and expressed as µg of dye per mg of protein in the tissue. Values are mean ± SD (n = 7 to 10). (*p < 0.05: compared with OIR and control group) (OIR: Oxygen-induced retinopathy).

• 

  Figure 2 Time-dependent effects of TA on vascular hyperpermeability in the retinas of OIR rats. At P16 the right eye received an intravitreal injection of TA (4 mg per eye) and the left eye received saline. Vascular permeability was measured 2 and 0, 2, 4, 6, 10 and 14 days before and after the injection. The Evans blue in the tissues was normalized by total protein concentration and expressed as µg of dye per mg of protein in the tissue. Values are mean ± SD (n = 7 to 10). (*p < 0.05: compared with TA- and saline-treated group) (TA: triamcinolone acetonide).

• 

  Figure 3 Expression of mRNAs analyzed by RT-PCR in the retina of both control and OIR rats and changes after TA treatment. This image is representative from six rats, showing PCR-amplified products of VEGF, HIF-1α, SDF-1, Occludin and ICAM-1, and β-actin as a loading control (TA: triamcinolone acetonide, OIR: oxygen-induced retinopathy).

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