J Korean Ophthalmol Soc.  2016 May;57(5):786-793. 10.3341/jkos.2016.57.5.786.

The Repeatability of Retinal Layer Thickness Measurements with Spectral-Domain Optical Coherence Tomography in Normal Eyes

• Kang MS
• Yu SY
• Kwak HW

• KMID: 2212722
• DOI: http://doi.org/10.3341/jkos.2016.57.5.786

Abstract

PURPOSE
To evaluate the repeatability of retinal layer thickness measurements in normal eyes imaged using spectral domain optical coherence tomography (SD-OCT).
METHODS
Sixty-eight eyes of 34 healthy subjects were enrolled in this study. Imaging was performed 4 times using 9 × 9 mm macular scans with SD-OCT (RS 3000 Advance HD OCT, NIDEK, Gamagori, Japan) at the same visit by an experienced examiner. After automatic retinal segmentation (layering) in 5 layers, the thickness of each layer was calculated. Macular thickness of 9 Early Treatment of Diabetic Retinopathy Study (ETDRS)-like regions was obtained. Repeatability for each of the 9 subfield areas was calculated by their repeatability coefficients and intraclass correlation coefficients (ICCs).
RESULTS
There was no significant difference in average retinal thickness and each retinal layer thickness between all measurements acquired by the experienced examiner. The ICCs of retinal layer thickness ranged from 0.826 to 0.847 for the ganglion cell layer + inner plexiform layer, inner nuclear layer + outer plexiform layer and outer nuclear layer + external limiting membrane in the fovea. The ICCs were greater than 0.909 for the other intra-retinal layers in all 9 ETDRS subfield thickness between all measurement pairs.
CONCLUSIONS
Excellent repeatability was observed for SD-OCT retinal segmented layer thickness measurements in healthy subjects.

Keyword

Automated segmentation; Intraclass correlation coefficients; Repeatability; Retinal layer thickness; Spectral domain optical coherence tomography

MeSH Terms

Diabetic Retinopathy
Ganglion Cysts
Membranes
Retinaldehyde*
Tomography, Optical Coherence*
Retinaldehyde

Figure

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  Figure 1. Early Treatment Diabetic Retinopathy Study (ETDRS) subfield (left eye). F = fovea; S1 = superior inner; T1 = temporal inner; I1 = inferior inner; N1 = nasal inner; S2 = superior outer; T2 = temporal outer; I2 = inferior outer; N2 = nasal outer.

• 

  Figure 2. Spectral domain optical coherence tomography (SD-OCT) automatic segmentation of the retinal layers in the macula. ILM = inner limiting membrane; RNFL = retinal nerve fiber layer; GCL = ganglion cell layer; IPL = inner plexiform layer; INL = inner nuclear layer; OPL = outer plexiform layer; ONL = outer limiting layer; ELM = external limiting membrane; IS/OS = inner segment/outer segment photoreceptor layer; RPE = retinal pigment epithelium.

Reference

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