J Reprod Endocrinol.  2013 Apr;5(1):18-26.

The Relationship Between Leptin and Adrenergic Receptors Genes Polymorphisms and Changes in Production of Osteoprotegerin and Soluble Receptor Activator of NF-kappaB by Whole Blood Cells after Hormone Therapy

• Park KE
• Lee HJ
• Kim H
• Ku SY
• Kim SH
• Choi YM
• Kim JG

Abstract

OBJECTIVES
To investigate the relationship between single nucleotide polymorphisms (SNPs) in leptin and adrenergic receptors genes, and the production of osteoprotegerin (OPG) and soluble receptor activator of NF-kappaB ligand (sRANKL) by whole blood cells (WBC) after hormone therapy (HT) in postmenopausal Korean women.
METHODS
The leptin receptor c.326A>G, c.668A>G, c.1968G>C SNPs, beta2 adrenergic receptor c.46A>G, c.79C>G SNPs, and beta3 adrenergic receptor c.190T>C SNP were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and Taqman assay in postmenopausal Korean women. The production of OPG and sRANKL by lipopolysaccharide (LPS)-stimulated WBC before (n=149) and after (n=73) estrogen-progestogen therapy of 6 months were also measured.
RESULTS
At baseline, the production of OPG and sRANKL by LPS-stimulated WBC, and in ratios of sRANKLx1,000/OPG were not different according to leptin and beta2 adrenergic receptors genetic polymorphisms, but the TT genotype of beta3 adrenergic receptor c.190T>C SNP showed significantly lower OPG production than non-TT genotype. Among SNPs measured, the beta2 adrenergic receptor c.46A>G SNP only was related with changes in the production of sRANKL by WBC after HT of 6 months. The production of sRANKL by WBC after HT decreased significantly in AA genotype of beta2 adrenergic receptor c.46A>G SNP, compared to other genotypes.
CONCLUSION
The beta3 adrenergic receptor c.190T>C SNP is related with the production of OPG by WBCs before HT, whereas the beta2 adrenergic receptor c.46A>G SNP affects the changes in the production of sRANKL by WBCs after HT.

Keyword

Adrenergic receptor; Hormone therapy; Leptin; Osteoprotegerin; Polymorphism

MeSH Terms

Blood Cells
Female
Genotype
Humans
Leptin
Osteoprotegerin
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Receptor Activator of Nuclear Factor-kappa B
Receptors, Adrenergic
Receptors, Leptin
Leptin
Osteoprotegerin
Receptor Activator of Nuclear Factor-kappa B
Receptors, Adrenergic
Receptors, Leptin