J Reprod Endocrinol.  2012 Apr;4(1):20-29.

The Relationship between Polymorphisms of Wnt Antagonists Genes and Production of Osteoprotegerin (OPG)-soluble receptor Activator of NF-kappaB Ligand (sRANKL) by Whole Blood Cells

Affiliations
  • 1Department of Obstetrics and Gynecology, Incheon Medical Center, Incheon, Korea.
  • 2Department of Obstetrics and Gynecology, National Cancer Center, Goyang, Korea.
  • 3Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
  • 4Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.

Abstract


OBJECTIVES
To investigate the association between polymorphisms of Wnt antagonists genes and the production of osteoprotegerin (OPG), and soluble receptor activator of nuclear factor-kappaB ligand (sRANKL) by whole blood cells (WBCs) in postmenopausal Korean women.
METHODS
The Dkk1 c.318A>G, Dkk2 c.437G>A, Dkk3 c.1003A>G polymorphisms and sFRP3 c.970c>G, sFRP4 c.958C>A, and c.1019G>A polymorphisms, and sFRP5 c.20G>C polymorphisms were analyzed by Taqman assay, DNA sequencing, and restriction fragment length polymorphism (RFLP) in 156 Korean postmenopausal women. The production of OPG and sRANKL by lipopolysaccharide-stimulated WBCs were also measured.
RESULTS
The production of OPG by lipopolysaccharide-stimulated WBCs was related with the Dkk1 c.318A>G polymorphism. The AA genotype of the Dkk1 c.318A>G polymorphism showed a higher production (P=0.04) of OPG by lipopolysaccharide-stimulated WBCs than GG genotype. No significant differences in the production of OPG and sRANKL by WBCs and their ratio were noted according to single polymorphisms of the sFRP genes and the haplotype genotypes of sFRP4 c.958C>A and c.1019G>A polymorphisms.
CONCLUSION
The Dkk1 c.318A>G polymorphism among Dkks and sFRPs polymorphisms measured affects the production of OPG by lipopolysaccharide-stimulated WBCs.

Keyword

Genetic polymorphism; Osteoprotegerin; Receptor activator of nuclear factor-kappaB ligand; Whole blood cells; Wnt antagonists

MeSH Terms

Blood Cells
Female
Genotype
Haplotypes
Humans
Osteoprotegerin
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Sequence Analysis, DNA
Osteoprotegerin
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
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