J Korean Ophthalmol Soc.  2007 Apr;48(4):573-588.

Proteomic Analysis of the Vitreous with Proliferative Diabetic Retinopathy

• Lee SJ
• Lee SH
• Park SH
• Lee SC
• Kwon OW

Abstract

PURPOSE: This study analyzed protein alterations between the normal vitreous and the vitreous with proliferative diabetic retinopathy by proteomics to find the proteins which may elicit diabetic retinopathy.
METHODS
Two-dimensional electrophoresis was used to make the protein map. Image analysis between the spots on each gels by a proteomics based approach were used to reveal vitreous protein alterations which may elicit proliferative diabetic retinopathy. MALDI-TOF/ESI-TOF mass spectrometry also was used to identify altered protein spots on the gel.
RESULTS
Of the 110 different spots on each gels, 36 different proteins were identified and among them 23 proteins were altered in the vitreous with proliferative diabetic retinopathy compared with normal vitreous. Nineteen proteins including alpha-1-antitrypsin, Ig G and A, and complement C3 and C4 were increased in the vitreous with proliferative diabetic retinopathy and 4 proteins includng pigment epithelium derived factor were decreased compared to the normal vitreous.
CONCLUSIONS
The authors found that pigment epithelium derived factor may be the key protein that induces the neovascularization in the vitreous with proliferative diabetic retinopathy. Increased levels of Ig G and A and C3 and C4 is thought to be related to the autoimmune inflammation in early diabetic microangiopathy. Furthermore, proteins such as alpha-1-antitrypsin may contribute to protective functions of the ischemic retinal cells.

Keyword

Diabetic retinopathy; Electrophoresis; MALDI-TOF; Protein; Vitreous

MeSH Terms

Complement C3
Diabetic Angiopathies
Diabetic Retinopathy*
Electrophoresis
Epithelium
Gels
Inflammation
Mass Spectrometry
Proteomics
Retinaldehyde
Complement C3
Gels
Retinaldehyde