J Korean Soc Plast Reconstr Surg.  1998 Oct;25(7):1226-1235.

The effects of silicone on mononuclear cell blastogenesis and il-1beta/tnf-alphasecretion of monocyte in human

• No authors listed

Abstract

Although there are numerous reviews of clinical and epidemiologic data, there has been no critical analysis of silicone immunology. The purpose of this study is to identify human celluar immune reaction to silicone through mononuclear cell blastogenesis as well as by measuring IL-1betaand TNF-alpha released from human monocyte/macrophage incubated with silicone in vitro. In the study, total 14 healthy volunteers participated in the experiment as blood donors. in the peripheral blood mononuclear cell blastogenesis assay, one control group and three experimental groups were designed. The three experimental groups were composed of a silicone treated group, a silicone/phytohemagglutinin treated group, and a phytohemagglutinin treated group. The peripheral blood mononuclear cells were isolated with the Ficoll-Hypaque gradient method, and they were incubated for 72 hours. The proliferation of the peripheral blood mononuclear cells was measured with the [3H]-thymidme uptake. In the cytokine assay of monocyte stimulated by silicone, human monocyte was isolated from the peripheral blood mononuclear cells through the magnetic cell sorting(MACS) method. One control group and three experimental groups were designed also in the experiment. The experimental groups were composed of a silicone treated group, a silicone/lipopolysaccharide treated group, and a lipopolysaccharide treated group. The monocytes of each group were incubated for 1,3,6,24 and 48 hours. The supernatants were preserved at -20degree C to measure IL-1betaand TNF-alpha with ELISA. Statistical analysis was done by two-way ANOVA and Scheffe test(p< 0.05). In the peripheral blood mononuclear cell blastogenesis study, no statistical difference was found either between the silicon treated group and the control group, or between the silicone/phytohemagglutinin treated group and the phytohemagglutinin treated group. The silicone treated group had higher IL-1betalevel than the control group 1 hour and 3 horus(p< 0.05), and the silicone/LPS treated group and had higher IL-1betalevel than the LPS treated group at 1 hour(p< 0.05). However the TNF-alphalevel had shown statistical difference neither between the silicone treated group and control group nor between the silicone/LPS treated group and the LPS treated group at any time.In conclusion, IL-1beta was released from the human monocyte/macrophage induced by silicione, and silicone did not induce peripheral blood mononuclear cell blastogenesis. It has been known that IL-1betacan induce the chemotaxis of T lymphocyte-subpopulation and T cell infiltration in tissue. Therefore the results of this experiments are regarded as an evidence of silicone-induced inflammation and tissue destruction such as rheumatic disease.

MeSH Terms

Allergy and Immunology
Blood Donors
Chemotaxis
Enzyme-Linked Immunosorbent Assay
Healthy Volunteers
Humans*
Inflammation
Lymphocyte Activation*
Monocytes*
Rheumatic Diseases
Silicones*
Tumor Necrosis Factor-alpha
Silicones
Tumor Necrosis Factor-alpha