J Korean Soc Plast Reconstr Surg.
2004 Nov;31(6):858-864.
Gene Expression Profiling of 1,2-Dimethylhydrazine-Stimulated Human Umbilical Vein Endothelial Cells
- Affiliations
-
- 1ABC Aesthetic Clinic, Busan, Korea.
- 2Department of Plastic and Reconstructive Surgery, College of Medicine, Pusan National University, Busan, Korea.
Abstract
- The purposes of this study are to establish standard model in which endothelial cell proliferations are induced by DMH stimulation in vitro, and to analyze the gene expressions of proliferative HUVECs using DNA chip technique which could evaluate the mechanisms of angiogenesis, and the development of vascular tumors. To perform the MTT assay in 96-well microplates, 104 cells were seeded in each well which were cultured in medium. On the third day, the cells were treated with 5 different concentrations of diluted DMH from 10 to 10-3 ng/ml. Five DMH-treated groups were compared with the control group which was not treated with DMH. The optical densities in each group were measured at the time of 0, 6, 12, 24, 36, 48, and 72 hours after DMH treatment. The same experiment was repeated 9 times. Statistically significant cell proliferations were observed in 1 and 10-1ng/ml group. The RNAs were isolated from HUVECs of control group and 1ng/ml DMH-treated group, and they were used to analyze the gene expressions using DNA chip technique. One hundred and seventy-seven genes(142 of up-retulated genes and 35 down-regulated genes) were identified, and several genes were associated with VEGF and FGF production. Also DMH could affect expression of genes that involve oncogenesis. Further study should be performed to evaluate the processes of angiogenesis and morphogenesis of vascular tumors, which could be utilized in the development of new therapeutic approaches.
MeSH Terms
-
Carcinogenesis
Dimenhydrinate
Dimethylhydrazines
Endothelial Cells
Gene Expression Profiling*
Gene Expression*
Human Umbilical Vein Endothelial Cells*
Humans*
Morphogenesis
Oligonucleotide Array Sequence Analysis
RNA
Umbilical Veins
Vascular Endothelial Growth Factor A
Dimenhydrinate
Dimethylhydrazines
RNA
Vascular Endothelial Growth Factor A
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Role of Protein Kinase C in Abnormal Proliferation of Vascular Endothelial Cell induced by 1,2-Dimethylhydrazine; Analysis of Isoform
- The Effect of VEGF (Vascular endothelial growth factor) on Endothelial Cells in Pathogenesis of Preeclampsia
- Protective Effect of Allium tuberosum Extract on Vascular Inflammation in Tumor Necrosis Factor-α-induced Human Vascular Endothelial Cells
- In Vitro Culture of Endothelial Cell and Smooth Muscle Cell for Studying Vascular Diseases
- Platelets Induce Proliferation of Human Umbilical Vein Endothelial Cells via CD154-CD40 Pathway Independently of VEGF
